长链非编码RNA FENDRR在急性胰腺炎患者血清中的表达及意义

Expression and significance of lncRNA FENDRR in serum of patients with acute pancreatitis

目的探究长链非编码RNA(lncRNA)叉头蛋白F1相邻非编码发育调控RNA(FENDRR)在急性胰腺炎(AP)患者血清中的表达水平及临床意义。方法选择2016年3月至2018年5月在北京大学深圳医院就诊的123例AP患者作为研究对象(设为AP组),另择同期在本院体检的52位健康志愿者作为对照组。采用实时荧光定量PCR(real-time qPCR)法检测患者血清中lncRNA FENDRR的相对表达量。比较轻症AP(MAP)组与重症AP(SAP)组、生存组与死亡组患者血清中lncRNA FENDRR的相对表达量。分析lncRNA FENDRR与AP病情严重程度及患者预后的关系。结果AP组患者血清lncRNA FENDRR的相对表达量为4.47±1.87,低于对照组(6.66±1.26),差异有统计学意义(P<0.05)。SAP组患者血清lncRNA FENDRR的相对表达量为3.14±1.22,低于MAP组(5.45±1.64),差异有统计学意义(P<0.05)。血清lncRNA FENDRR诊断AP患者病情严重程度的ROC曲线下面积(AUC)为0.869(95%CI0.796~0.923),敏感度为71.15%,特异度为90.14%。单因素及多因素logistic回归分析结果显示,血小板(PLT)、国际标准化比值(INR)、血清钙、乳酸和lncRNA FENDRR与AP患者的预后关系密切。单指标评估AP患者预后结果指出,PLT的评估效能最大AUC=0.813(95%CI0.733~0.878),其次为lncRNA FENDRRAUC=0.784(95%CI0.701~0.853),乳酸的评估效能最小AUC=0.673(95%CI0.583~0.755)。PLT、INR、血清钙、乳酸和lncRNA FENDRR联合检测评估AP患者预后的效能高于各项单独检测的效能。结论血清lncRNA FENDRR的表达水平越低,提示AP患者病情越严重,预后不良风险越高。lncRNA FENDRR可能是AP疾病严重程度和预后评估的潜在生物标志物。

Objective An attempt is made in this paper to investigate the expression and significance of long non-coding RNA(lncRNA)forkhead box-F1 adjacent non-coding developmental regulatory RNA(FENDRR)in serum of patients with acute pancreatitis(AP).Methods A total of 123 AP patients who were admitted to Peking University Shenzhen Hospital from March 2016 to May 2018 were assigned to the experimental group while 52 healthy subjects were assigned to the control group.The relative expression of lncRNA FENDRR in serum was detected by real-time fluorescence quantitative PCR(real-time qPCR).The relative expression of lncRNA FENDRR in serum of patients in the mild AP(MAP)group and the severe AP(SAP)group,and that in the survival group and the death group were compared.The relationship between lncRNA FENDRR and AP severity and prognosis was analyzed.Results The expression of serum lncRNA FENDRR in the AP group was lower than that in the control group((4.47±1.87)versus(6.66±1.26),P<0.05).The expression of serum lncRNA FENDRR in the SAP group was lower than that in the MAP group((3.14±1.22)versus(5.45±1.64),P<0.05).The ROC AUC of serum lncRNA FENDRR in diagnosing the severity of AP was 0.869(95%CI:0.796 to 0.923),and the sensitivity and specificity were 71.15%and 90.14%respectively.The univariate and multivariate logistic regression analysis showed that platelet(PLT),international normalized ratio(INR),serum calcium,lactic acid and lncRNA FENDRR were closely related to the prognosis of AP patients.The single-index diagnosis of AP patients with prognosis,PLT diagnostic efficiency is the largest(AUC=0.813(95%CI:0.733 to 0.878)),followed by lncRNA FENDRR(AUC=0.784(95%CI:0.701 to 0.853)),and the diagnostic efficacy of lactic acid minimum(AUC=0.673(95%CI:0.583 to 0.755)).The combined detection of PLT,INR,serum calcium,lactic acid,and lncRNA FENDRR is more effective in assessing the prognosis of AP patients than that of individual detection.Conclusions The lower the expression level of serum lncRNA FENDRR,the more serious the condition of AP patients,and the higher the risk of poor prognosis.lncRNA FENDRR may be a potential biomarker for the severity and prognosis of AP.