基于转录组测序的ATPR诱导NB4细胞分化过程中lncRNA的初步鉴定与分析

Preliminary identification and analysis of lncRNA during differentiation of NB4 cells induced by ATPR based on transcriptome sequencing

目的为了探究新型维甲酸衍生物4-氨基-2-三氟甲基苯基维甲酸酯(ATPR)诱导人急性早幼粒细胞白血病NB4细胞分化的潜在作用机制。方法分别用ATPR和全反式维甲酸(ATRA)诱导NB4细胞分化,然后用基因芯片技术对处理后的细胞进行长链非编码RNA(lncRNA)和mRNA表达谱分析。结果与ATRA组比较,有775个lncRNA和904个mRNA在ATPR组被筛选出,且顺式调控基因分析了一些关键lncRNA的染色体定位。反式分析结果表明相关转录因子能调节lncRNA的表达。结论lncRNA在ATPR诱导NB4细胞分化过程中起到重要的调节作用。

Objective To explore the potential mechanism of differentiation of human acute promyelocytic leukemia NB4 cells induced by a new retinoic acid derivative 4-amino-2-trifluoromethyl-phenyl retinoate(ATPR).Methods After the differentiation of NB4 cells induced by ATPR and all trans retinoic acid(ATRA),we analyzed the long-chain non-coding RNA(lncRNA)and mRNA expression profile of the treated cells with gene chip technology.Results The results showed that 775 lncRNAs and 904 mRNAs were screened in the ATRA group compared with the ATRA group,and the chromosomal location of some key lncRNAs was analyzed by cis regulatory genes.The results of trans analysis showed that the related transcription factors could regulate the expression of lncRNA.Conclusion In conclusion,lncRNA plays an important role in the differentiation of NB4 cells induced by ATPR.