针刺捻转补泻法对自发性高血压大鼠 海马丙二醛、谷胱甘肽过氧化物酶含量的影响

Effects of reinforcing-reducing method by twirling of acupuncture on contents of malondialdehyde and glutathione peroxidase in hippocampus of spontaneously hypertensive rats

目的探讨针刺捻转补泻法对自发性高血压大鼠海马组织形态及海马中丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)含量的影响。方法18只自发性高血压大鼠按照随机数字表法分为三组:泻法组、补法组与模型组,每组6只。6只WKY大鼠为空白组。所有大鼠均为SPF级9周龄雄性大鼠。适应性喂养1周后,泻法组和补法组采用相应手法针刺双侧太冲穴14 d,1次/d。模型组与空白组进行与泻、补法组同等位置的抓取固定刺激。第15天,采用HE染色法观察海马组织,硫代巴比妥酸法检测MDA含量,比色法检测GSH-Px含量。结果HE染色结果显示,空白组细胞结构完整,形态规则,细胞间隙正常,多数细胞核深染,核仁清晰,未见变性坏死。与空白组比较,模型组染色明显变浅,出现核固缩、细胞溶解碎裂、空泡样变性和凋亡细胞。与模型组比较,泻法组和补法组均可见细胞轮廓较清晰,细胞染色加深,核仁清晰,细胞结构基本正常。模型组的MDA含量高于空白组,GSH-Px含量低于空白组,差异有高度统计学意义(P<0.01)。泻法组、补法组MDA含量均低于模型组,GSH-Px含量均高于模型组,差异有统计学意义(P<0.05或P<0.01)。结论针刺捻转补泻法能通过调节自发性高血压大鼠海马中MDA和GSH-Px的表达水平,抑制氧化反应,减轻氧自由基对其组织损伤,这可能是针刺捻转补泻法对高血压海马损伤的保护机制之一。

Objective To explore the effects of reinforcing-reducing method by twirling of acupuncture on contents of malondialdehyde(MDA) and glutathione peroxidase(GSH-Px) in hippocampus of spontaneously hypertensive rats.Methods A total of 18 spontaneously hypertensive rats were divided into three groups by random number table method:reducing method group, reinforcing method group, and model group, with six rats in each group. Six WKY rats were served as blank group. All rats were SPF grade, nine-week-old male rats. After one week of acclimatization, reducing method group and reinforcing method group were intervened with corresponding manipulation of reducing and reinforcing on bilateral tàichōng once a day for 14 days. Model group and blank control group received the same grasping and fixation stimulation at the same points of reinducing method group and reinforcing method group. Hippocampus samples were collected on the 15 th day, and the morphology of hippocampus was observed by HE staining, MDA content was detected by thiobarbituric acid method and GSH-Px content was detected by colorimetry. Results HE staining results showed that the cells in the blank group had complete structure, regular morphology, normal intercellular space, most nuclei were deeply stained, nucleoli were clear, and no denatured necrosis was observed. Compared with the blank group, the staining in the model group was significantly lighter, and nuclear pyknosis, cell lysis and fragmentation, vacuolar degeneration and apoptotic cells were observed.Compared with the model group, the cell contour was clear, the cell staining was deep, the nucleoli were clear, and the cell structure was basically normal in both the reducing method group and reinforcing method group. MDA content in model group was higher than that in blank group, while GSH-Px content in model group was lower than that in blank group, and the differences were highly statistically significant(P < 0.01). MDA contents in reducing method group and reinforcing method group were lower than those in model group, the contents of GSH-Px in the reducing method group and reinforcing method group were higher than those in the model group, and the differences were statistically significant(P < 0.05 or P <0.01). Conclusion Reinforcing-reducing method by twirling of acupuncture can regulate the expression level of MDA and GSH-Px in the hippocampus of spontaneously hypertensive rats, inhibit the oxidative reaction, and reduce the damage of oxygen radicals to the hippocampus of spontaneously hypertensive rats, which may be one of the protective mechanisms of reinforcing-reducing method by twirling of acupuncture on hypertensive hippocampal injury.