胃癌患者血清β-肌动蛋白和G蛋白偶联受体-4的表达及临床意义

Expression and clinical significance of serumβ-actin and G protein-coupled receptor-4 in patients with gastric cancer

目的研究胃癌患者血清β-肌动蛋白(β-actin)及G蛋白偶联受体-4(LGR4)的表达及其在胃癌中的诊断价值。方法选取2018年2月—2020年6月呼和浩特市第二医院诊治的103例胃癌患者(观察组)及同时期的胃良性肿瘤患者52例(对照组),采用酶联免疫吸附法分别测定血清中β-actin与LGR4的表达水平,分析其与临床病理参数的关系,绘制ROC曲线分析β-actin与LGR4对胃癌的诊断。结果观察组患者血清中β-actin与LGR4的表达水平均高于对照组,差异均有统计学意义(均P<0.05),血清β-actin、LGR4在低~中分化、TNM分期Ⅲ~Ⅳ期及无淋巴结转移胃癌患者的表达高于高分化、Ⅰ~Ⅱ分期和有淋巴结转移患者(P<0.05)。ROC曲线分析结果显示:血清中β-actin与LGR4的截断值为3.21、47.62 pg/mL时,诊断胃癌的ROC-AUC分别为0.747、0.782,联合检测为0.869。结论胃癌患者血清中β-actin、LGR4的表达水平升高,且在低~中分化、TNM分期Ⅲ~Ⅳ期及无淋巴结转移患者中高表达,二者联合检测有可能作为诊断胃癌的分子标志物。

Objective To study the expression of serumβ-actin and G protein coupled receptor-4(LGR4)in patients with gastric cancer and their diagnostic value in gastric cancer.Methods A total of 103 cases of gastric cancer patients(observation group)and 52 cases of benign gastric cancer at the same period(control group)treated in Hohhot Second Hospital were selected from February 2018 to June 2020.The expression levels of serumβ-actin and LGR4 were measured by enzyme-linked immunosorbent assay and the relationship between the two indexes and clinicopathological parameters were analyzed,the ROC curve was used to analyze the diagnosis of gastric cancer.Results The expression levels ofβ-actin and LGR4 in serum of observation group were higher than those of control group,with statistical significance(all P<0.05).The expression of serumβ-actin and LGR4 in patients with low to medium differentiation,Ⅲ-Ⅳstage in TNM stage and without lymph node metastasis were higher than those in patients with high differentiation,Ⅰ-Ⅱstage and with lymph node metastasis(P<0.05).ROC curve analysis showed that when the cut-off values ofβ-actin and LGR4 in serum were 3.21 and 47.62 pg/mL,the ROC-AUC for diagnosis of gastric cancer was 0.747 and 0.782,respectively,and the combined detection was 0.869.Conclusion The expression levels ofβ-actin and LGR4 in gastric cancer were increased,and highly expressed in patients with low to moderate differentiation,Ⅲ-Ⅳstage in TNM stage and no lymph node metastasis.The combined detection of β-actin and LGR4 may be used as molecular markers for the diagnosis of gastric cancer.