武汉市一般人群血清中全氟及多氟烷基化合物浓度及其影响因素研究

Concentration of perfluoroalkyl and polyfluoroalkyl substances in serum and their influencing factors in general population in Wuhan,China

目的了解武汉市一般人群血清中全氟及多氟烷基化合物(PFASs)浓度,探索血清中PFASs浓度水平的影响因素。方法以在武汉市某医院进行一般体检的人群为研究对象(n=67),通过超高效液相色谱-三重四级杆质谱(UPLC-TQ/MS)联用仪测定外周血清中12种PFASs浓度,采用多元线性回归分析方法分析一般人口学特征和血清中PFASs浓度的关联性。结果武汉市一般人群血清中12种PFASs的平均浓度水平为11.60 ng/mL。全氟辛烷磺酸及其盐类(PFOS)和全氟辛酸及其盐类(PFOA)的贡献率较大,分别为45.03%和31.86%。男性人群血清中PFOS、PFOA、全氟己基磺酸(PFHxS)、全氟壬酸(PFNA)等主要化合物的浓度高于女性,其β值(95%可信区间)分别为0.708(0.313,1.104)、0.518(0.069,0.967)、0.724(0.388,1.059)和0.684(0.399,0.968)。同时,发现血清中PFOS、PFHxS、PFNA浓度和年龄呈正相关,其β值(95%可信区间)分别为0.062(0.042,0.081)、0.035(0.019,0.052)和0.030(0.016,0.045)。结论性别和年龄对血清中PFASs浓度有影响,在进行人体PFASs暴露评估中应考虑这些因素。

Objective To investigate the concentration of perfluoroalkyl and polyfluoroalkyl substances(PFASs)in the serum of the general population in Wuhan,and to discover the influencing factors of the concentrations of PFASs in serum.Methods Total of 67 people who had physical examination in a certain hospital in Wuhan was selected as the study object.The concentration of 12 kinds of PFASs in peripheral serum were determined by ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-TQ/MS).The correlation between general demographic characteristics and PFASs concentration in serum was discovered by multiple linear regression analysis.Results The average concentration of the 12 kinds of PFASs in the serum of the general population in Wuhan was 11.60 ng/mL,which was lower than those in previous studies.Perfluorooctane sulphonate(PFOS)and perfluoro octanoic acid(PFOA)were the main contributors,accounting for 45.03%and 31.86%respectively.The concentration of main compounds including PFOS,PFOA,perfluorohexanesulfonic acid(PFHxS),and perfluorononanoic acid(PFNA)in the serum of male population was significantly higher than that of female population,with theβvalue and 95%confidence interval(95%Cl)of 0.708(0.313,1.104),0.518(0.069,0.967),0.724(0.388,1.059)and 0.684(0.399,0.968)respectively.At the same time,PFOS,PFHxS and PFNA in serum were positively correlated with age,with theβvalue and 95%Cl of 0.062(0.042,0.081),0.035(0.019,0.052)and 0.030(0.016,0.045)respectively.Conclusion Gender and age have influence on PFASs concentration in serum,and these factors should be taken into account for human exposure assessment of PFASs.