基于mRNA-Seq筛选贵州白山羊抗捻转血矛线虫病相关下调表达基因的研究

Study of the screening of down-regulated genes related to resistance to Haemonchus contortus in Guizhou white goats based on mRNA-Seq

为了研究贵州白山羊抗捻转血矛线虫病的抗病机理并挖掘相关重要调控因子,试验采用转录组测序的方法对自然感染捻转血矛线虫的贵州白山羊血液进行研究,利用生物信息学方法分析试验组和对照组血液中差异表达基因并构建相关的基因调控通路。结果表明:共获得121个差异表达基因,试验组有75个下调表达基因。这些下调表达基因大部为线粒体相关基因(如ND1、ND4和COX3等),其中ND1、ND4基因表达量极显著下调(P<0.01),而COX3基因表达量显著下调(P<0.05)。对下调表达基因进行GO功能富集分析及KEGG信号通路分析,这些下调表达基因共分为三大类38组,共发现8条通路,其中基因富集最为显著的是遗传信息处理、能量代谢和氧化磷酸化等与线粒体相关的信号通路,线粒体功能障碍主要表现在能量代谢紊乱等方面。说明这些基因表达量或功能发生变化的原因可能是捻转血矛线虫感染导致宿主贫血及皱胃局部发炎,从而参与炎症调控过程。

In order to study the disease resistance mechanism of Guizhou white goats against Haemonchus contortus and to discover relevant important regulatory factors, the experiment used transcriptome sequencing method to study the blood of Guizhou white goats naturally infected with Haemonchus contortus;differentially expressed genes in the blood of the experimental group and the control group were analyzed using bioinformatics methods, and related gene regulatory pathways were constructed. The results showed that a total of 121 differentially expressed genes were obtained, and there were 75 down-regulated genes in the experimental group. Most of down-regulated genes were mitochondrial-related genes(ND1,ND4,COX3). The expression of ND1,ND4 genes was extremely significantly down-regulated(P<0.01),and the expression of COX3 gene was significantly down-regulated(P<0.05). The GO function enrichment analysis and KEGG signal pathway analysis of down-regulated expressed genes were carried out. These down-regulated expressed genes were divided into three categories and 38 groups. A total of 8 pathways were found. Among them, the most significant gene enrichment was genetic information processing, energy metabolism and oxidative phosphorylation, which is mitochondria-related signal pathways. Mitochondrial dysfunction was mainly manifested in energy metabolism disorders. The results suggested that the changes in the expression or function of these genes may be caused by the infection of Haemonchus contortus which led to host anemia and local abomasum inflammation, and participated in the inflammation regulation process.