不同条件对弓形虫卵囊孢子化进程的影响

Effect of different conditions on the sporulation process of Toxoplasma gondii oocysts

为了研究不同条件对弓形虫卵囊孢子化进程的影响,试验用新鲜生猪肝喂猫,从粪便中分离弓形虫卵囊,在有氧和无氧条件下,于不同温度[4℃、室温(18~22℃)、25℃和37℃]、硫酸溶液浓度(1%、2%、4%)和pH值(1~14)情况下培养,在培养至第2,4,6,8,10,12,14,16,18,20,22,24天时镜检观察弓形虫卵囊孢子化情况。结果表明:在喂食猫的第6天成功分离到弓形虫卵囊。在有氧条件下,4℃时弓形虫卵囊培养至第24天未孢子化;室温时pH值为4,5,10,12,13,14培养至第2天开始孢子化;25℃时pH值为4培养至第2天开始孢子化;37℃时pH值为1,2,3,4,9,12,13培养至第2天开始孢子化。在无氧条件下,4℃时pH值为1和3弓形虫卵囊培养至第12天开始孢子化;室温时硫酸溶液浓度为1%、2%和pH值为1,2,3,4,5,11,12,13,14培养至第14天开始孢子化;25℃时硫酸溶液浓度为1%、4%和pH值为1培养至第12天开始孢子化;37℃时培养至第24天未孢子化。说明25℃是弓形虫卵囊孢子化的最适温度,4℃可抑制弓形虫卵囊孢子化,氧气有利于弓形虫卵囊孢子化,pH值和硫酸溶液浓度对弓形虫卵囊孢子化的影响较小。

In order to study the effect of different conditions on the sporulation process of Toxoplasma gondii oocysts, the experiment was conducted to feed the cats with fresh pig liver, and to separate the oocysts of Toxoplasma gondii from the feces;under aerobic and anaerobic conditions, it was incubated at different temperatures [4 ℃,room temperature(18~22 ℃),25 ℃ and 37 ℃], sulfuric acid solution concentration(1%,2%,4%) and pH values(1-14);on the 2 nd, 4 th, 6 th, 8 th, 10 th, 12 th, 14 th, 16 th, 18 th, 20 th, 22 th, and 24 th day of culture, the sporulation of Toxoplasma gondii oocysts was observed under microscopy. The results showed that the Toxoplasma gondii oocysts were successfully isolated on the 6 th day of feeding the cat. Under aerobic conditions, Toxoplasma gondii oocysts were cultured at 4 ℃ until it did not sporulate on the 24 th day;at room temperature, with the pH value of 4,5,10,12,13,and 14,it began to sporulate on the second day;at 25 ℃ and the pH value of 4,it began to sporulate on the second day;at 37 ℃ and the pH value of 1,2,3,4,9,12,13,it began to sporulate on the second day. Under anaerobic conditions, at 4 ℃ and the pH values of 1 and 3,Toxoplasma gondii oocysts began to sporulate on the 12 th day;at room temperature, the concentration of sulfuric acid solution of 1%,2%,and the pH value of 1,2,3,4,5,11,12,13,14,it began to sporulate on the 14 th day;at 25 ℃,sulfuric acid solution concentration of 1%,4%,and the pH value of 1,it began to sporulate on the 12 th day;at 37 ℃,it was not sporulated on the 24 th day. The results suggested that 25 ℃ was the optimum temperature for the oocyst sporulation of Toxoplasma gondii;4 ℃ can inhibit the oocyst sporulation of Toxoplasma gondii;oxygen was beneficial to the oocyst sporulation of Toxoplasma gondii;pH value and sulfuric acid solution concentration had low effect on the oocyst sporulation of Toxoplasma gondii.