甜菜阿拉伯聚糖包埋姜黄素工艺的响应面优化

Response surface optimization of the encapsulation technology of curcumin in sugar beet arabinan

本研究首先采用高效液相色谱-高分辨质谱(RP-HPLC-DAD/ESI-TOF-MS)分析甜菜阿拉伯聚糖(SBA)中阿魏酸及其衍生物等疏水基团的含量;再研究SBA包埋姜黄素,制备水包油型(O/W)乳液的工艺,考察温度、搅拌器输入功率和SBA浓度等因素对SBA包埋姜黄素效果的影响。结果表明,SBA中FA含量为2079.8±72.7μg/g,8-O-4’-diFA含量为18.0±1.0μg/g。响应面法优化得到SBA包埋姜黄素的工艺为:温度50℃,搅拌器输入功率0.24kW·h,SBA浓度5.68mg/mL。在此优化条件下,模型预测的最大响应为1.93(%),实际试验得到的平均值为2.02±0.10(%)(n=3),验证了响应面方法模型的有效性。

In this study,the content of ferulic acid(FA)and its derivatives in sugar beet arabinose(SBA)was firstly analyzed by RP-HPLC-DAD/ESi-TOF-MS.Then the process of curcumin encapsulated by(SBA)was investigated,the effect of preparation conditions,including temperature,stirrer input power and concentration of SBA on the encapsulation capacity of curcumin(ECC)in O/W emulsion were evaluated.The results showed that SBA contained 2079.8±72.7μg/g of FA and 18.0±1.0μg/g of 8-O-4’-diFA.The experiment was carried out under the optimal conditions(within the experimental range):temperature of 50℃,stirrer input power of 0.24kW·h,and concentration of SBA 9.16mg/mL.Under the optimal conditions,the model predicted a maximum response of 1.93(%).A mean value of 2.02±0.10(%)(n=3),obtained from real experiments,demonstrated the validation of the response surface methodology(RSM)model.