高效液相色谱法测定草果中4种酚酸含量

Determination of 4 kinds of phenolic acids content from the Amomumtsao-ko by high performance liquid chromatography

目的建立高效液相色谱法测定草果中4种酚酸类成分的分析方法。方法采用高效液相色谱法,选用C18色谱柱(250 mm×4.6 mm,5μm),以甲醇和0.02 mol/L的稀磷酸溶液为流动相进行梯度洗脱,对草果中4种主要酚类成分(原儿茶酸、原儿茶醛、对羟基苯甲酸、香草酸)的含量进行同时分析测定;检测波长为270 nm,流量为1.0 mL/min,柱温为30℃。结果原儿茶酸在0.04~0.36 mg(r=0.99988)、原儿茶醛在0.04~0.36 mg(r=0.99973),对羟基苯甲酸在0.16~1.44 mg(r=0.99993)、香草酸在0.04~0.36 mg(r=0.99993)内呈良好的线性关系。平均加样回收率分别为93.3%、97.4%、97.1%、95.5%,RSD分别为2.95%、0.99%、1.34%、1.54%;测得样品中各成分的含量范围分别为,原儿茶酸9.12~17.42 mg/kg、原儿茶醛7.11~15.51 mg/kg、对羟基苯甲酸22.67~57.08 mg/kg、香草酸7.92~14.21 mg/kg。结论该方法灵敏度高、稳定性强,可较好地控制草果的质量品质。

Objective To establish a method for the determination of 4 phenolic acids from the Amomumtsao-ko by high performance liquid chromatography.Methods The content of 4 phenolic acids in Amomumtsao-ko(protocatechuic acid,protocatechualdehyde,p-hydroxybenzoic acid,vanillic acid)was analyzed and determined simultaneously by high performance liquid chromatography on a C18 column(250 mm×4.6 mm,5μm),using methanol and 0.02 mol/L diluted phosphoric acid as mobile phases for gradient elution.The detection wavelength was 270 nm,the flow rate was 1.0 mL/min,and the column temperature was 30℃.Results Protocatechuic acid,protocatechualdehyde,p-hydroxybenzoic acid,vanillic acid showed good linear relationships in the range of 0.04-0.36 mg(r=0.99988),0.04-0.36 mg(r=0.99973),0.16-1.44 mg(r=0.99993)and 0.04-0.36 mg(r=0.99993),respectively.The average recoveries were 93.3%,97.4%,97.1%and 95.5%,RSDs were 2.95%,0.99%,1.34%and 1.54%,respectively.The content of each index component in different samples was protocatechuic acid 9.12-17.42 mg/kg,protocatechualdehyde 7.11-15.51 mg/kg,p-hydroxybenzoic acid 22.67-57.08 mg/kg,vanillic acid 7.92-14.21 mg/kg.Conclusion This method is highly sensitive with strong specificity and it can be used efficiently in the quality control of Amomumtsao-ko.