miR-711在介导结肠癌干细胞生物学行为恶化中的作用

Role of miR-711 in the biological behavior deterioration of colon cancer stem cells

目的探讨miR-711对结肠癌干细胞细胞侵袭、转移、增殖及上皮间质转化的作用。方法采用流式细胞仪从人结肠癌细胞株HT-29中分选肿瘤干细胞。miR-711-mimics、miR-NC转染结肠癌干细胞。qRT-PCR检测各组细胞miR-711 mRNA表达;MTT检测细胞增殖能力;Transwell小室实验检测细胞迁移、侵袭能力;Western Blot检测E-cadherin、N-cadherin、Vimentin蛋白量表达。结果结肠癌细胞株HT-29细胞CD44+/CD133+百分比最高(29.90%);miR-711过表达组细胞miR-711水平高于空载组和空白组(P<0.05);miR-711过表达组细胞增殖、侵袭及迁移能力低于空载组、空白组,差异有统计学意义(P<0.05);miR-711过表达组E-cadherin蛋白水平高于空载组、空白组,N-cadherin、Vimentin蛋白水平低于空载组、空白组,差异均有统计学意义(P均<0.05)。结论过表达miR-711可抑制结肠癌干细胞侵袭、转移、增殖及上皮间质转化。

Objective To investigate the effect of miR-711 on invasion, invasion, proliferation and epithelial-mesenchymal transition of colon cancer stem cells.Methods Colon cancer stem cells were isolated from human colon cancer cell lines HT-29 by flow cytometry.miR-711-mimics and miR-NC were transfected into colon cancer stem cells.qRT-PCR was used to detect the expression of miR-711 mRNA.MTT was used to detect cell proliferation.Transwell test was used to detect cell migration and invasion.Western blot was used to detect the protein expression of E-cadherin, N-cadherin, Vimentin.Results The percentage of CD44+/CD133+in colon cancer cell line HT-29 was the highest(29.90%).The expression of miR-711 in over-expression group was higher than that in empty group and blank group(P<0.05).The proliferation, invasion ans migration ability of over-expression group was lower than those of empty group and blank group(P<0.05).The expression level of E-cadherin protein in over-expression group was higher than that of empty group and blank group(P<0.05).The expression level of N-cadherin, Vimentin protein in over-expression group were lower than those of empty group and blank group(P all<0.05).Conclusion Up-regulation of miR-711 can inhibit the invasion, invasion, proliferation and epithelial mesenchymal-transition of colon cancer stem cells.