miRNA101对人牙囊细胞成骨分化的影响

Effects of miRNA101 on osteogenic differentiation of human dental follicle cells

目的:研究miRNA101在人牙囊细胞(DFCs)成骨分化过程中的作用及探讨其调节成骨分化的相关机制。方法:体外培养DFCs,转染miRNA101诱导miRNA101表达,提取总RNA和miRNA,RT-PCR检测miRNA-101表达情况及成骨细胞分化相关基因ALP、OCN、Runx2、SATB2的水平,采用LabAssayTM ALP检测试剂盒检测碱性磷酸酶ALP活性。结果:miRNA-101能够调控DFCs的成骨分化。体外转染miRNA101模拟物后,碱性磷酸酶活性增加,并且成骨分化相关基因及成骨转录因子SATB2表达上调。结论:体外诱导DFCs成骨分化后miRNA101的表达升高。体外转染增加miRNA101的表达能够促进人类牙囊细胞的成骨分化。

Objective This study was to research the effect of miRNA101 on osteogenic differentiation and the possible molecular mechanisms of it promotes the osteogenic differentiation.Methods DFCs were cultivated in vitro and expression of miRNA101 was induced by miRNA101-mimic transfection and the gene expression of osteogenic transcription factors such as ALP、OCN、Runx2、SATB2 was obtained by real-time RT-PCRs.LabAssayTM ALP test kit was used for detecting ALP activity.Results miRNA-101 could regulates osteogenic differentiation of DFCs.After miRNA101-mimic transfection the alkaline phosphatase was increased and the gene expression of typical osteogenic transcription factors such as SATB2 was up-regulated.Conclusion miRNA101 increased in vitro induction of osteogenic differentiation of DFCs and the increasement of miRNA101 could promote the osteogenic differentiation of human dental follicle cells.