五味子酒对小鼠睡眠及记忆功能的影响

Effect of Schisandra wine on sleep and memory function of mice

目的研究五味子酒对小鼠睡眠及记忆功能的影响。方法50只昆明雄性小鼠随机分为空白组,阳性对照组,五味子酒低剂量(6.25 mg/kg)、中剂量(12.5 mg/kg)、高剂量组(25 mg/kg)组,连续灌胃30 d。睡眠实验包括直接睡眠实验,戊巴戊巴比妥钠阈下剂量对睡眠的影响和延长戊巴比妥钠睡眠时间试验;记忆实验采用水迷宫进行定位航行和空间探索实验;酶联免疫吸附试验测定神经递质中多巴胺(DA)、五羟色胺(5-HT)、五羟吲哚乙酸(5-HIAA)的含量。结果与空白组比较,五味子酒组小鼠睡眠发生率提高,戊巴比妥钠致睡眠持续期延长,定位航行实验中逃避潜伏期逐渐缩短,空间探索实验中穿越平台次数逐渐增多,东北象限停留时间增多,下丘脑中神经递质DA、5-HT和5-HIAA含量增加,以上结果具有统计学意义(P<0.05)。结论五味子酒有改善小鼠睡眠以及记忆力的功能。

Objective To study the effect of Schisandra wine on sleep and memory function of mice.Methods Fifty male Kunming mice were randomly divided into blank group,positive control group,low dose(6.25 mg/kg),medium dose(12.5 mg/kg)and high dose(25 mg/kg)of Schisandra chinensis wine groups,and they were given gavage continuously for 30 days.Sleep test included direct sleep test,subthreshold dose of pentobarbital sodium on sleep and prolonged sleep time of pentobarbital sodium.Memory test used water maze for place navigation test and spatial probe test.Enzyme linked immunosorbent assay was used to determine the contents of dopamine(DA),5-hydroxytryptamine(5-HT)and 5-hydroxyindoleacetic acid(5-HIAA)in neurotransmitters.Results Compared with the blank group,the incidence of sleep was increased,the sleep duration of pentobarbital sodium prolonged,the escape latency was shortened in place navigation test,the number of crossing platforms increased in spatial probe test and the stay time in northeast quadrant increased,and the contents of neurotransmitters DA,5-HT and 5-HIAA in hypothalamus were increased in Wuweizi wine group(P>0.05).Conclusion Schisandra wine can improve sleep and memory of mice.