LncRNA-ATB对乳腺癌细胞增殖、迁移和凋亡的影响

The effect of lncRNA-ATB on proliferation,migration and apoptosis of breast cancer

目的:研究lncRNA-ATB对乳腺癌细胞的增殖、迁移和凋亡的影响。方法:培养乳腺癌细胞MCF-7、MDA-MB-231,将两种细胞各分为5组:空白组、si-NC组、si-ATB-1组、si-ATB-2组、si-ATB-3组,实时荧光定量PCR检测lncRNA-ATB的表达量,验证转染效率及筛选最适干扰序列进行后续实验。采用克隆平板形成实验检测细胞增殖情况,transwell细胞迁移实验检测细胞迁移能力,流式细胞术检测细胞凋亡率。结果:两种乳腺癌细胞MCF-7、MDA-MB-231中,空白组及si-NC组lncRNA-ATB水平明显高于si-ATB1、2、3组,si-ATB-1组表达量最低,选为最适干扰组进行后续实验。与空白组及si-NC组比较,si-ATB组细胞克隆形成能力及迁移明显降低,而si-ATB组细胞凋亡率明显高于空白组及si-NC组。结论:下调lncRNA-ATB的表达可抑制乳腺癌细胞的增殖及迁移,并促进癌细胞凋亡。

Objective To investigate the effect of lncRNA-ATB on the proliferation,migration and apoptosis of breast cancer cells.Methods Breast cancer cell MCF-7 and MDA-MB-231 were cultured,and the two types of cells were divided into 5 groups:blank group(no treatment),si-NC group(transfected negative sequence),si-ATB(transfected specific interfering RNA,designed and synthesized 3 specific siRNA interference sequences,a total of 3 groups),by using real-time PCR to detect the expression of lncRNA-ATB,to verify the transfection efficiency and screen the most suitable interference sequence for subsequent experiments.Colony formation assay was used to detect the ability of cell proliferation,transwell migration assay was used to evaluate the migratory ability,and examined the cell apoptosis rate by flow cytometry.Results In the two breast cancer cells MCF-7 and MDA-MB-231,the level of lncRNA-ATB in the blank group and the si-NC group are significantly higher than that in the si-ATB1,2,and 3 groups.The expression level of si-ATB-1 is the lowest in these groups,and it was selected as the most suitable interference group for subsequent experiments.Compared with the blank group and si-NC group,the abilities of colony formation and migratory in si-ATB group were significantly reduced,while the cell apoptosis rate of the si-ATB group was significantly higher than blank group and si-NC group.Conclusion Down-regulating the expression of lncRNA-ATB can inhibit breast cancer cell proliferation and migration,and promote cancer cell apoptosis.