静松灵对小型猪不同脑区氨基酸类神经递质的影响

Effects of Xylazole on Amino Acid Neurotransmitter in Different Encephalic Regions of Miniature Pigs

麻醉药发挥麻醉作用的机制可能是通过影响特定受体使动物不同脑区氨基酸类神经递质的含量发生改变实现的。因此,本试验通过观察静松灵对小型猪不同脑区氨基酸类神经递质的影响,探讨静松灵产生麻醉作用的可能机制。本试验将20只小型猪随机分为4组,对照组肌内注射生理盐水[0.25 mL/(kg·bw)],其他组肌内注射静松灵[0.25 mL/(kg·bw)],通过反相高效液相色谱(RP-HPLC)测定不同时间点各脑区谷氨酸(GLU)、天冬氨酸(ASP)、甘氨酸(GLY)、γ-氨基丁酸(GABA)的含量变化。结果显示,静松灵会引起小型猪小脑内兴奋性神经递质GLU和ASP在麻醉期含量下降,大脑和丘脑抑制性神经递质GLY和GABA在诱导期含量上升。根据试验结果,推测静松灵的中枢麻醉机制可能与抑制小脑内兴奋性神经递质释放和促进大脑、丘脑内抑制性神经递质释放相关。

The mechanism by which anesthetics exert their anesthetic effects might be by influencing specific receptors to change the content of amino acid neurotransmitters in different brain regions of animals.In order to study the effects of xylazole anesthetic on amino acid neurotransmitters in different brain regions of miniature pigs,20 Bama pigs in three months of age were randomly divided into four groups.After the pigs in control group were treated with intramuscular injection normal saline(NS)and pigs in other groups were treated with intramuscular injection of xylazole[0.25 mL/(bw·kg)],the contents of glutamate(GLU),aspartic acid(ASP),glycine(GLY)andγ-aminobutyric acid(GABA)in different brain regions were detected by reversed-phase high performance liquid chromatography(RP-HPLC)at different time points.The results indicated that xylazole could induce the decrease of excitatory neurotransmitter GLU and ASP in cerebellum of miniature pigs during anesthesia,and the increase of inhibitory neurotransmitter GLY and GABA in brain and thalamus during induction.Therefore,we speculate that the central anesthesia mechanism of xylazole may be related to inhibiting the release of excitatory neurotransmitters in cerebellum and promoting the release of inhibitory neurotransmitters in brain and thalamus.