枸杞多糖对顺铂诱导小鼠睾丸支持细胞凋亡的影响

Effects of lycium barbarum polysaccharides on cis-dichlorodiamineplatinum(II)-induced apoptosis in mouse testis sertoli cells TM4

目的:研究枸杞多糖(lycium barbarum polysaccharides,LBP)对顺铂(cis-dichlorodiamineplatinum,CDDP)诱导小鼠睾丸支持细胞(TM4)凋亡的影响并探讨其可能机制。方法:体外培养TM4细胞,四甲基偶氮噻唑蓝(MTT)法检测LBP对CDDP诱导TM4细胞生存率的影响,Western blot检测LBP对CDDP诱导TM4细胞凋亡相关基因Bcl-2、Bax和Caspase-3蛋白表达的影响,流式细胞仪检测细胞凋亡率的变化。结果:与Control组相比,CDDP组TM4细胞凋亡显著增加,细胞内抑凋亡基因Bcl-2表达量显著降低,促凋亡基因Bax表达量及Caspase-3表达量显著升高;与CDDP组相比,CDDP+LBP组TM4细胞凋亡显著减少,细胞内抑凋亡基因Bcl-2表达量显著升高,促凋亡基因Bax表达量及Caspase-3表达量显著降低。结论:LBP作用于CDDP诱导的TM4细胞,能够通过增强细胞内Bcl-2的表达,抑制Bax及Caspase-3的表达来阻抑因CDDP诱导的TM4细胞凋亡,进而减轻CDDP对TM4细胞造成的损伤。

AIM:To explore the effect of Lycium barbarum polysaccharides(LBP)on cis-dichlorodiamineplatinum(II)(CDDP)-induced apoptosis in mouse testis sertoli cells TM4 and its possible mechanism.METHODS:TM4 cells were cultured in vitro,the effect of LBP on the survival rate of TM4 cells induced by CDDP was detected by MTT assay,the effect of LBP on the expression of apoptosis related genes Bcl-2,Bax and Caspase-3 in TM4 cells induced by CDDP was detected by Western blot,and the change of cell apoptosis rate was detected by flow cytometry.RESULTS:Compared with control group,TM4 cell apoptosis was significantly increased in CDDP group,the expression of anti-apoptotic gene Bcl-2 and pro-caspase-3 in proenzyme state were significantly decreased,the expression of pro-apoptotic gene Bax and caspase-3 were significantly increased.Compared with CDDP group,the apoptosis of TM4 cells in CDDP+LBP group was significantly decreased,the expression levels of anti-apoptotic genes Bcl-2 and Pro-Caspase3 were significantly increased,the expression levels of pro-apoptotic gene Bax and Caspase-3 were significantly decreased.CONCLUSION:LBP,by acting on CDDP induced TM4 cells,can inhibit CDDP induced TM4 cell apoptosis by enhancing the expression of Bcl-2 and inhibiting the expression of Bax and Caspase-3,thus alleviating the damage caused by CDDP to TM4 cells.