HPLC同时测定不同产地石吊兰中的4种黄酮类成分

Simultaneous determination of four flavonoids of Lysionotus pauciflorus by HPLC

采用反相HPLC法同时测定石吊兰药材中吊石苷C、去甲氧基苏打基亭、5-羟基-6,8,4'-三甲氧基-7-O-β-D-葡萄糖黄酮苷和石吊兰素等4种黄酮类成分的含量。采用Cosmosil 5 C18-AR-II色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇(A)-0.1%甲酸水(B)溶液,梯度洗脱,流速1.0 mL·min^(-1),柱温30℃,进样量20μL,波长329 nm。4种黄酮类成分均能达到基线分离,在线性范围内的线性关系良好(r^(2)>0.9991),精密度、重复性、稳定性均满足分析方法的要求;加样回收率为98.82%~101.08%,RSD为1.36%~1.79%。14批不同产地石吊兰样品中4种黄酮类成分的含量存在显著差异。所用方法精度高、简便,可作为石吊兰的质量控制方法。

To simultaneous determine four flavonoids of lysioside,demethoxysudachjtin,5-hydroxy-6,8,4'-trimethoxy flavone-7-O-β-D-glucopyranosyl,and nevadensin in Lysionotus pauciflorus by HPLC.The method was also applied to the analysis of the contents differences of four investigated flavonoids comparing 14 L.pauciflorus samples in China.The analysis was performed on a Cosmosil 5C18-AR-II(250 mm×4.6 mm,5μm)column at 30℃.Gradient elution was conducted with acetonitrile(solvent A)and 0.1%formic acid(solvent B)at a flow rate of 1.0 mL·min^(-1)with 329 nm detection wavelength.Baseline separation of four flavonoids was achieved using the present methods.Excellent linear behavior was observed over the investigated concentration range(r^(2)>0.9991).The precision,repeatability and stability fulfilled the requirements of methodology.Recovery and RSDs of four flavonoids were from 98.82%to 101.08%,and from 1.36%to 1.79%,respectively.The results showed that the contents of four flavonoids from 14 L.pauciflorus samples were significantly different.The optimal method is simple,convenient and with a good repeatability and high precision.It can be used for the quality control of L.pauciflorus.