基于CS-Au NPs@ GO/GCE构建电化学免疫传感器对甲胎蛋白的检测

Detection of Carcinoembryonic Antigen by Electrochemical Immunosensor based on CS-Au NPs@ GO/GCE

利用氧化石墨烯(GO)较大的比表面积可以,采用电化学方法在其表面电沉积壳聚糖(CS)-金纳米粒子(Au NPs),制备CS-Au NPs@ GO复合材料。利用Au NPs对蛋白质的吸附作用及CS分子的-OH与蛋白质分子的-NH_(2)和-COOH结合作用,构建电化学免疫传感器,用于甲胎蛋白(AFP)的检测。在最优的条件下,运用电化学交流阻抗(EIS)和差分脉冲伏安法(DPV)等对传感器进行电化学表征。结果表明,构建的传感器表现出低的检测限和良好的抗干扰性,可以较好地用于AFP的快速检测。

With the large specific surface area of graphene oxide(GO),chitosan(CS)-gold nanoparticles(Au NPs)were decorated on the surface of GO by electrodeposition method to prepare CS-Au NPs@GO composites.Based on the characteristics of Au NPs that can adsorb proteins,as well as the bonding between CS molecules and protein molecules.An electrochemical immunosensor constructed for the detection of alpha-fetoprotein(AFP).Under the optimal conditions,electrochemical impedance(EIS)and differential pulse voltammetry(DPV)were used to characterize the sensor.The results showed the low detection limit and good anti-interference,which could be used for the rapid detection of AFP.