基于网络药理学探讨藿香正气方治疗妊娠期新型冠状病毒肺炎的作用机制

Exploration of Mechanism of Huoxiang Zhengqi Recipe in Treating Novel Coronavirus Pneumonia in Pregnancy Based on Network Pharmacology

目的采用网络药理学方法分析藿香正气方治疗妊娠期新型冠状病毒肺炎的作用机制,探究其多成分—多靶点—多通路的相关关系。方法运用中药系统药理学分析平台(TCMSP)筛选藿香正气方中的活性成分,SwissTargetPrediction服务器收集成分靶点,人类基因数据库(GeneCards)获取妊娠期新型冠状病毒肺炎靶点,将成分靶点与疾病靶点映射,获取共有靶点。STRING数据库构建蛋白互作(PPI)网络,用Cytoscape3.3.0软件进行拓扑参数分析,得到核心靶点,并构建"药物—成分—靶点—疾病"网络。DAVID数据库对核心靶点进行GO富集分析和KEGG通路分析。结果筛选得到藿香正气方中84个活性成分,1007个成分靶点,疾病靶点236个,两者交集靶点72个。涉及丝裂原活化蛋白激酶1(MAPK1)、白细胞介素6(IL-6)、MAPK3、MAPK8、肿瘤坏死因子(TNF)、表皮生长因子受体(EGFR)、IL-2等31个核心靶点。GO富集分析显示448个条目,主要集中于细胞凋亡的调控、氧化应激反应、对细胞因子刺激的反应等过程。KEGG富集分析有52条通路,主要参与Toll样受体信号通路、NOD样受体信号通路、ErbB信号通路、血管内皮生长因子(VEGF)信号通路和癌症、免疫、凋亡等相关通路调控。结论藿香正气方的有效成分可通过作用于MAPK1、IL-6、MAPK3、MAPK8、TNF、EGFR等靶点调节多条信号通路,发挥对妊娠期新型冠状病毒肺炎的治疗作用。

Objective To analyze the mechanism of Huoxiang Zhengqi Recipe in treating novel coronavirus pneumonia in pregnancy by using network pharmacology and explore the relationship between its multiple components,targets and pathways.Methods The active components in Huoxiang Zhengqi Recipe were screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).The component targets were obtained by SwissTargetPrediction server.The targets in novel coronavirus pneumonia in pregnancy were identified from GeneCards database.The component targets and disease targets were mapped to obtain the common targets.The protein-protein interaction network was established by STRING database.Cytoscape 3.3.0 software was used for network topology parameters analysis to obtain core targets.The"medicine-components-targets-disease"network was constructed.GO function enrichment analysis and KEGG pathway enrichment analysis were performed through DAVID.Results A total of 84 active components of Huoxiang Zhengqi Recipe,1007 component targets and 236 disease targets were obtained.There were 72 intersection targets,involving 31 core targets such as mitogen-activated protein kinase 1(MAPK1),interleukin-6(IL-6),MAPK3,MAPK8,tumor necrosis factor(TNF),epidermal growth factor receptor(EGFR),IL-2,etc.GO function enrichment analysis revealed 448 items,including regulation of apoptosis,response to oxidative stress and response to cytokine stimulus,etc.KEGG pathway enrichment screened 52 signal pathways,including Toll-like receptor,NOD-like receptor,ErbB,VEGF signaling pathways and cancer,immunization,apoptosis and other related pathways.Conclusion The active components of Huoxiang Zhengqi Recipe may have a therapeutic effect on novel coronavirus pneumonia in pregnancy through the action on targets such as MAPK1,IL-6,MAPK3,MAPK8,TNF,EGFR,etc.and regulating many signaling pathways.