分散固相萃取-UPLC-MS/MS法测定动物肝脏中左旋咪唑的残留量

Determination of Levamisole Residues in Animal Liver by Dispersive Solid Phase Extraction-UPLC-MS/MS

研究了采用1%冰乙酸-乙腈溶液分散固相萃取(QuEChERS),建立了超高效液相色谱-串联质谱法测定动物肝脏中左旋咪唑残留量的分析方法。样品提取后,用无水硫酸钠(Na_(2)SO_(4))及氨基(NH2)吸附剂净化,多反应监测扫描模式(MRM)分析,采用外标法定量。实验结果表明,左旋咪唑在10~200μg/L线性范围内具有良好的线性关系,相关系数(r^(2))为:0.9996。测定低限(LOQ)为10μg/kg。试验以空白样品为基体做加标回收,测得左旋咪唑的回收率在71.3%~105.5%,相对标准偏差(n=6)为5.2%~8.1%。满足动物肝脏中左旋咪唑残留量的快速分析要求。

The study was established an analytical method for the determination of levamisole residues in animal liver by dispersive solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry. The samples were extracted with 1% glacial acetic acid-acetonitrile solution, purified with anhydrous sodium sulfate(Na_(2)SO_(4)) and amino(NH2) adsorbents, analyzed by multi-reaction monitoring scanning mode(MRM), and quantified by external standard method. Levamisole had a good linear relationship in the linear range of 10-200 μg/L, and the correlation coefficient(r^(2)) was 0.9996. The lower limit of determination(LOQ) was 10 μg/kg. In the test, the blank sample was used as the matrix to do standard recovery. The recovery rate of levamisole was 71.3%-105.5%, and the relative standard deviation(n=6) was 5.2%-8.1%. Meet the requirements of rapid analysis of levamisole residues in animal livers.