anti-IL-33对类风湿关节炎成纤维样滑膜细胞增殖与凋亡的影响

The Effect of anti-IL-33 on the Proliferation and Apoptosis of Fibroblast-like Synovial Cells in Rheumatoid Arthritis

目的探讨抗白细胞介素-33抗体(anti-IL-33)对类风湿关节炎(RA)成纤维样滑膜细胞(FLS)增殖与凋亡的影响及其在RA疾病中的缓解及治疗作用。方法培养人FLS,实验分组如下:正常对照组、TNF-α(+)对照组、TNF-α(+)+anti-IL-33(50 ng/ml)组、TNF-α(+)+甲氨蝶呤(1μg/ml)组。观察RA-FLS的形态结构,并采用CCK-8法测定各组细胞的增殖水平,流式细胞仪检测各组细胞的细胞凋亡率。结果形态学检测显示,anti-IL-33能缓解经TNF-α处理后导致的FLS细胞变圆、间隙增加。CCK8法检测显示,与对照组比较,TNF-α(+)对照组细胞活力增加(P<0.05);与TNF-α(+)对照组比较,TNF-α(+)+anti-IL-33组和TNF-α(+)+甲氨蝶呤组细胞活力降低(P<0.05)。凋亡检测显示,与对照组比较,TNF-α(+)对照组细胞凋亡降低(P<0.05);与TNF-α(+)对照组比较,TNF-α(+)+anti-IL-33组和TNF-α(+)+甲氨蝶呤组细胞凋亡率增高(P<0.05)。结论 anti-IL-33可抑制RA-FLS增殖并诱导其凋亡,可为RA的治疗提供方向。

Objective To investigate the effect of anti-IL-33 antibody(anti-IL-33)on the proliferation and apoptosis of rheumatoid arthritis(RA)fibroblast-like synovial cells(FLS)and its mitigation and therapeutic effects in RA disease.Methods Cultivating human FLS,the experimental groups are as follows:Normal control group,TNF-α(+)control group,TNF-α(+)+anti-IL-33(50 ng/ml)group,TNF-α(+)+methotrexate(1μg/ml)group.Observing the morphology and structure of RA-FLS,and use CCK-8 method to determine the proliferation level of each group of cells,and flow cytometry to detect the apoptosis rate of each group of cells.Results Morphological examination showed that anti-IL-33 can alleviate the rounding and gap increase of FLS cells caused by TNF-αtreatment.CCK8 method detection showed that compared with the control group,the cell viability of the TNF-α(+)control group increased(P<0.05);Compared with the TNF-α(+)control group,the cell viability of the TNF-α(+)+anti-IL-33 group and the TNF-α(+)+methotrexate group were decreased(P<0.05).Apoptosis detection showed that compared with the control group,the apoptosis of the TNF-α(+)control group was reduced(P<0.05);Compared with the TNF-α(+)control group,the apoptosis rate of the TNF-α(+)+anti-IL-33 group and the TNF-α(+)+methotrexate group was increased(P<0.05).Conclusion anti-IL-33 can inhibit the proliferation of RA-FLS and induce its apoptosis,which can provide a direction for the treatment of RA.