绝经后骨质疏松症患者血清IL-31、IL-33表达变化

Changes of serum IL-31 and IL-33 levels in postmenopausal patients with osteoporosis

目的分析白细胞介素-31(IL-31)和白细胞介素-33(IL-33)在绝经后骨质疏松症(PMOP)患者血清中的表达变化,探讨IL-31和IL-33与PMOP的关联性。方法选取绝经后女性共146例,使用双能X线骨密度仪(DXA)检测受试者的骨密度,根据骨密度将受试者分为三组:骨量正常组、低骨量组和骨质疏松组。测定血钙(Ca)、磷(P)、甲状旁腺激素(PTH)、25-羟基维生素D([25(OH)D])、IL-31、IL-33及骨转换指标血清骨特异性碱性磷酸酶(BALP)、血清1型原胶原N-端前肽(P1NP)、血清抗酒石酸酸性磷酸酶(TRACP-5b)、血清1型胶原羧基端交联肽(β-CTX)的水平。比较不同骨量的绝经后女性血清IL-31、IL-33及骨转换指标的差异。结果三组受试者的血清IL-31浓度随着骨密度的降低而增加,而血清IL-33浓度随着骨密度的降低而下降,差异均有统计学意义(P<0.05)。多项式Logistic回归分析结果显示,低骨量组中IL-33(OR:0.987,95%CI:0.974~0.999,P=0.030)差异有统计学意义,骨质疏松组中IL-31(OR:1.019,95%CI:1.009~1.028,P<0.001)、IL-33(OR:0.978,95%CI:0.960~0.995,P=0.013)差异有统计学意义,IL-33的OR值随着骨密度的降低呈下降趋势。提示IL-31和IL-33为PMOP的可能影响因素。结论IL-31为PMOP的可能危险因素,而IL-33为PMOP的可能保护因素,提示IL-31和IL-33在PMOP发生过程中起重要作用。

Objective To analyze the changes of interleukin-31(IL-31)and interleukin-33(IL-33)levels in the serum of postmenopausal osteoporosis(PMOP)patients,and to explore the correlation between PMOP and IL-31/IL-33.Methods A total of 146 postmenopausal women were selected and their bone density was detected with double-energy X-ray absorptiometry(DXA).According to the bone density,the patients were divided into three groups:normal group,low bone mass group and osteoporosis group.Calcium(Ca),phosphorus(P),parathyroid hormone(PTH),25-hydroxyvitamin D([25(OH)D]),IL-31,IL-33,bone-specific alkaline phosphatase(BALP),type 1 procollagen N-terminal propeptide(P1NP),anti-tartrate acid phosphatase(TRACP-5b),andβ-cross linked C-telopeptide of type 1 collagen(β-CTX)were evaluated.The differences in IL-31,IL-33 and bone turnover markers among the three groups were compared.Results In the three groups,the level of IL-31 showed an upward trend with the decrease of bone density,while the level of IL-33 reduced with the decrease of bone density(P<0.05).Multivariate Logistic regression analysis showed that in the low bone mass group,IL-33(OR:0.987,95%CI:0.974-0.999,P=0.030)was statistically significant,while in the osteoporosis group,IL-31(OR:1.019,95%CI:1.009-1.028,P<0.001)and IL-33(OR:0.978,95%CI:0.960-0.995,P=0.013)were statistically significant.The OR value of IL-33 showed a downward trend with the decrease of bone mineral density,indicating that IL-31 and IL-33 were possible influencing factors of PMOP.Conclusion IL-31 is a possible risk factor for PMOP,while IL-33 is a possible protective factor for PMOP,suggesting that IL-31 and IL-33 play an important role in the pathogenesis of PMOP.