lncRNA DLEU2转录调控因子的生物信息学分析

Bioinformatics analysis of lnc RNA DLEU2 transcriptional regulatory factors

目的探讨lncRNA DLEU2转录调控因子的结构特征及其与lncRNA DLEU2结合位点。方法利用生物信息学工具分析lncRNA DLEU2转录调控因子的理化性质、结构、跨膜区、信号肽和亚细胞定位,预测转录因子与lncRNA DLEU2的结合位点。结果等电点分析表明转录调控因子CTCF、MAX、ELF1、YY1、USF1等电点小于7.0,POLR2A、FOXA1、BHLHE40、CEBPB、ATF3等电点大于7.0;脂溶指数分析显示,上述10个转录调控因子的脂溶指数均小于100,均为疏水性蛋白;不稳定指数分析结果显示,本研究中10个转录调控因子的不稳定指数大于40,均为不稳定蛋白;上述所有转录调控因子二级结构均由α-螺旋、无规则卷曲和扩展链结构3种形式组成;上述转录调控因子中并未发现跨膜蛋白和信号肽结构;亚细胞定位分析发现,除YY1定位为细胞质中,其他转录因子均位于细胞核;同时预测出上述10个转录调控因子与lncRNA DLEU2的结合位点。结论lncRNA DLEU2转录调控因子呈现多样性特征,可能有助于对lncRNA DLEU2转录表达调控机制的深入研究,研究结果将有助于开展基于lncRNA DLEU2相关癌症治疗药物的筛选。

Objective To investigate the structural characteristics of lncRNA DLEU2 transcription factor and its transcriptional binding sites with lncRNA DLEU2.Methods Bioinformatics tools were used to analyse the physical and chemical properties,structure,transmembrane region,signal peptide and subcellular localisation of lncRNA DLEU2 transcriptional regulatory factors and to predict the transcriptional binding sites between transcriptional factors and lncRNA DLEU2.Results Isoelectric point analysis showed that the isoelectric points of transcriptional regulatory factors CTCF,MAX,ELF1,YY1 and USF1 were less than 7.0.The isoelectric points of POLR2 A,FOXA1,BHLHE40,CEBPB and ATF3 were more than 7.0.Lipolysis index analysis showed that the liposolubility index of the above 10 transcriptional regulatory factors were less than 100,indicating that they were hydrophobic proteins.The results of instability index analysis showed that the instability index of 10 transcriptional regulatory factors in this study were greater than 40,indicating that they were unstable proteins.All the secondary structures of the above transcriptional regulatory factors were composed ofα-helix,irregular coiling and extended chain structure,and no transmembrane protein and signal peptide structures were found in the above transcriptional factors.Subcellular localisation analysis showed that YY1 was located in the cytoplasm,and other transcription factors were located in the nucleus.At the same time,the 10 transcriptional regulatory factors and lncRNA DLEU2 transcriptional binding sites were predicted.Conclusion The diversity of lncRNA DLEU2 transcriptional regulatory factors may contribute to the further study of the regulation of lncRNA DLEU2 transcriptional expression,and the results will be helpful for the screening of lncRNA DLEU2 related cancer therapeutic drugs.