花生转录因子LEC1的表达特性分析

Analysis on Expression of the Transcription Factor LEC1 in Peanut

LEC1是调控植物胚形成和发育的重要转录因子。本研究根据已获得的花生AhLEC1基因序列,利用原核表达分析和实时荧光定量PCR(qRT-PCR)技术初步验证其在花生中的生物学功能。结果表明:①原核表达分析显示,获得了分子量为25 kD左右的目的蛋白条带,与预期大小一致。②组织表达分析显示,AhLEC1基因在花生的花及果针中微量表达,在荚果以及种子发育过程有明显的时序表达特性。AhLEC1基因在荚果发育过程中出现先增后降的趋势,在种子发育的前期表达量高,后期逐渐降低。③非生物胁迫表达分析显示,AhLEC1基因在低温、高盐以及干旱处理的花生叶片中表达量明显上调,表明AhLEC1基因可能参与花生对非生物胁迫的抗性调控。

LEC1 is an important transcription factor regulating the formation and development of plant embryo.In this study,based on the obtained peanut AhLEC1 gene sequence,the prokaryotic expression analysis and real-time fluorescence quantitative PCR(qRT-PCR)technology were used to preliminarily verify its biological function in peanut.The results showed that:①Prokaryotic expression analysis showed that a target protein band with a molecular weight of about 25 kD was obtained,which was consistent with the expected size.②Tissue expression analysis showed that the AhLEC1 gene was slightly expressed in peanut flower and peg,and had obvious temporal expression characteristics during pod and seed development process.The AhLEC1 gene showed a trend of increasing at first and then decreasing during the pod development.The expression level of AhLEC1 gene was high in the early stage of seed development and gradually decreased in the later stage.③Abiotic stress expression analysis showed that the expression of AhLEC1 gene was significantly up-regulated in peanut leaves treated with low temperature,high salt and drought,indicating that the AhLEC1 gene may be involved in the regulation of peanut resistance to abiotic stress.