黄芩苷对肺炎支原体感染肺上皮细胞增殖的影响

Impact of the Baicalin on Proliferation of M.pneumoniae-Infecting Lung Epithelial Cells in Vitro

目的:探讨黄芩苷对肺炎支原体感染A549上皮细胞增殖和细胞形态的影响。方法:采用MTT法、划痕实验、细胞克隆技术和免疫细胞化学染色等方法测定黄芩苷对感染肺炎支原体A549上皮细胞增殖作用,HE染色观察细胞形态变化。结果:黄芩苷在4~512 mg/L浓度范围内无细胞毒性。与模型对照组相比,黄芩苷各给药组存活细胞数增多,细胞增殖率显著升高(P<0.05),划痕长度缩短,细胞集落形成率升高(P<0.05),PCNA蛋白表达量增多(P<0.05)。HE染色见细胞结构形态较好,细胞膜清晰完整,细胞密度大,凋亡细胞减少。结论:黄芩苷对肺炎支原体感染肺上皮细胞具有细胞增殖促进作用。

Objective:To investigate the effects of Baicalin on the proliferation and morphology changes of A549 cells infected with M.pneumoniae.Methods:MTT assays,Scratch Wound Assays,Clone forming test,Immunostaining and HE stain were used to observed the effects of Baicalin on A549 cells proliferation infected with M.pneumoniae and the morphology changes.The morphological changes of cells were observed by HE staining.Results:Baicalin had no cytotoxicity in the concentration range of 4~512 mg/L.In contrast to the model group,the number of live cells in Baicalin group was increased,cell increment rate was increased significantly(P<0.05),scratch length was shortened,colony formation rate wasincreased(P<0.05),PCNA expression was increased(P<0.05).The structure and morphology of the cells were intact,the cell membrane was clear,the cell density was high and the apoptotic cells were reduced by HE staining.Conclusion:Baicalin can promote the proliferation of lung epithelial cells infected with M.pneumoniae.