子宫内膜异位症患者腹腔液中TNF-αIL-1β和ATG9A的表达水平及其相关性分析

Expressions of TNF-αIL-1βATG9A in endometriosis patients’peritoneal fluid and its correlation

目的探讨子宫内膜异位症(EMS)患者腹腔液中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和自噬相关蛋白9A(ATG9A)的表达水平及3者间的相关性。方法选取2017年1月至2019年12月于安徽医科大学第二附属医院妇科收治且经术后组织病理学诊断为EMS的患者85例为观察组,同期另选取非EMS的妇科患者80例为对照组,其中85例EMS患者依据修正EMS分期分为Ⅰ~Ⅱ期20例和Ⅲ~Ⅳ期65例。采用酶联免疫吸附法(ELISA)检测两组研究对象及不同分期EMS患者腹腔液中TNF-α、IL-1β及ATG9A水平,通过Person相关分析TNF-α、IL-1β与ATG9A的相关性。结果观察组腹腔液中TNF-α、IL-1β水平高于对照组,ATG9水平低于对照组,差异均有统计学意义(t=17.818、12.861、9.992,P<0.001);Ⅲ~Ⅳ期EMS患者腹腔液中TNF-α、IL-1β水平高于Ⅰ~Ⅱ期EMS患者,ATG9A水平低于Ⅰ~Ⅱ期EMS患者,差异均有统计学意义(t=131.691、210.807、59.443,P<0.001)。Person相关分析结果显示,TNF-α、IL-1β水平与ATG9呈负相关(r=-0.568、-0.597,P<0.001)。结论TNF-α、IL-1β及ATG9水平与EMS患者疾病严重程度相关。

Objective To explore the expressions of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and autophagy related protein 9A(ATG9A)in endometriosis(EMS)patients’peritoneal fluid,and to explore the correlation among the three factors.Methods A total of 85 EMS patients confirmed by postoperative histopathology who were admitted to the Second Hospital of Anhui Medical University from January 2017 to December 2019 were enrolled and served as the observation group.Meanwhile,a total of 80 no-EMS women were served as control group.According to the modified EMS staging,85 patients were divided into stageⅠ~Ⅱ(20 cases)and stageⅢ~Ⅳ(65 cases).The expressions of TNF-α,IL-1β,and ATG9A in two groups were detectedby enzyme linked immunosorbent assay(ELISA),and the correlation between TNF-α,IL-1βand ATG9A was assessedby Pearson analyse.Results The levels of TNF-αand IL-1βin the peritoneal fluid of the observation group were higher than those of the control group,while the level of ATG9A was lower than that of the control group,bothwith statistical significance(t=17.818,12.861,9.92,P<0.001).The levels of TNF-αand IL-1βin peritonal fluid of patients withⅢ~Ⅳperiod were higher than those of patients withⅠ~Ⅱperiod,and the level of ATG9A was lower than that of patients withⅠ~Ⅱperiod,with statistical significance(t=131.691,210.807,59.443,P<0.001).Person correlation analysis showed that the levels of TNF-αand IL-1βwere negatively correlated with the level of ATG9A(r=-0.568,-0.597,P<0.001).Conclusions The levels of TNF-α,IL-1βand ATG9A arecorrelated with the severity of EMS.