龙眼核多酚对LPS诱导的ALI小鼠肺组织的保护作用及机制

Protective effect of longan seed polyphenols on lung tissue in mice with LPS-induced ALI and its mechanism

目的分析龙眼核多酚对脂多糖(LPS)诱导的ALI小鼠肺组织的保护作用及相关机制。方法将32只小鼠随机分为4组,每组8只,分为对照组、模型组、地塞米松组及龙眼核多酚组,其中除了对照组,剩余3组小鼠建立性肺损伤小鼠模型,对照组、模型组注射等体积生理盐水,地塞米松组、龙眼核多酚组分别注射等体积地塞米松、龙眼核多酚;比较4组小鼠炎性水平、氧化应激反应。结果对照组小鼠肺组织结构完整,肺泡腔内无渗出物,肺间质正常,肺泡间隔无增厚;模型组肺组织病理平均分(12.51±3.48)分,地塞米松组平均分(4.81±1.63)分,龙眼核多酚组平均分(5.02±0.65)分,地塞米松、龙眼核多酚组高于模型组(P <0.05);而两组比较无显著差异(P> 0.05)。对照组肺组织湿重/干重比值、炎性反应指标明显低于其他3组(P <0.05),而地塞米松、龙眼核多酚组较模型组下降(P <0.05),但地塞米松、龙眼核多酚组比较(P> 0.05)。对照组SOD表达高于其他3组,MDA表达低于其他3组(P <0.05),而地塞米松、龙眼核多酚组SOD表达高于模型组,MDA表达低于模型组(P <0.05),但地塞米松、龙眼核多酚组比较(P> 0.05)。地塞米松、龙眼核多酚组HMGB1、NF-κB表达均低于模型组(P <0.05)。结论龙眼核多酚可减轻因LPS诱导的ALI小鼠的炎症反应及氧化应激反应,缓解肺组织病理状态。

Objective To analyze the protective effect of longan seed polyphenols on lung tissue in mice with lipopolysaccharide(LPS)-induced ALI and its related mechanism. Methods A total of 32 mice were randomized into four groups, including the control group, model group, dexamethasone group and longan seed polyphenol group, eight mice for each group. In addition to the control group, a lung injury mouse model was established for the other three groups. The control group and model group were injected with the same volume of normal saline;the dexamethasone group and longan seed polyphenol group were injected with the same volume of dexamethasone and longan seed polyphenols respectively. The inflammatory level and oxidative stress response were compared among the four groups. Results The control group had intact lung tissue structure, no exudates from alveolar space, normal lung interstitium,and no thickened alveolar septum. The average histopathological score was(12.51 ±3.48) in the model group,(4.81 ±1.63) in the dexamethasone group, and(5.02±0.65) in the longan seed polyphenol group;the score in the dexamethasone group and longan seed polyphenol group was lower than that in the model group(P < 0.05), and there was no difference between the dexamethasone group and longan seed polyphenol group(P > 0.05). The ratio of wet weight to dry weight and inflammatory response indicators in the control group were significantly lower than those in the other three groups(P < 0.05);the dexamethasone group and longan seed polyphenol group had decreased values compared with the model group(P < 0.05);there was no difference between the dexamethasone group and longan seed polyphenol group(P > 0.05). The control group had a higher SOD expression level and a lower MDA expression level compared with the other three groups(P < 0.05). The dexamethasone group and longan seed polyphenol group had a higher SOD expression level and a lower MDA expression level compared with the model level(P < 0.05);there was no difference between the dexamethasone group and longan seed polyphenol group(P > 0.05). The HMGB1 and NF-κB expression levels in the dexamethasone group and longan seed polyphenol group were lower than those in the model group(P < 0.05). Conclusion Longan seed polyphenols can reduce the inflammatory response and oxidative stress response of mice with LPS-induced ALI and alleviate the pathological state of lung tissue.