PK7300全自动血型仪在血型检测中的应用分析

Application of PK7300 automatic blood grouping analyzer in blood grouping test

目的分析PK7300全自动血型仪在血型检测中的应用价值。方法使用奥林巴斯PK7300全自动血型仪检测献血者血液样本ABO及RhD血型,对判定失败样本进行手工复核,将异常血型及初筛Rh阴性血型样本送血型参比室鉴定。结果2016—2019年共检测402649份样本,血型分析仪一次性判读成功399841份(99.30%),判读失败2808份(0.70%);送检Rh D血型鉴定样本2730份,确认阴性2644份(96.85%),阳性86份(3.15%);送检ABO正反定型不符样本101份,鉴定显示血型正常44份,AB亚型26份,含MNSs-抗-M抗体31份;送检单独O细胞凝集反应样本24份,鉴定显示血型正常7份,含MNSs-抗-M抗体14份,冷自身抗体2份,温自身抗体1份。结论PK7300血型仪能够检出一定数量的亚型和含MNSs-抗-M抗体的样本,具有操作简单、自动化程度高、准确率高和使用试剂量少等特点,可提升血型检测的工作效率和检测质量,适用于大批量血型检测。

Objective To analyze the application value of PK7300 automatic blood grouping analyzer in blood grouping identification.Methods Blood specimens from blood donors were analyzed by Olympus PK7300 blood grouping analyzer to identify the ABO and RhD blood groups.Samples with failed interpretation were retested by manual method.Abnormal blood types or initially screened as Rh-negative specimens were sent to the Blood Group Reference Laboratory of our center for further identification.Results A total of 402649 blood specimens during 2016 to 2019 were analyzed.In sum,there were 399841(99.30%)samples successfully identified at the first time,and 2808(0.70%)failed.A total of 2730 RhD samples were sent for further identification,including 2644(96.85%)Rh-negative samples,and 86(3.15%)Rh-positive ones.There were 101 samples with ABO discrepancies,among which 44 were identified as normal ABO blood group,26 as AB subtypes,and 31 contained MNSs-anti-M antibodies.Out of 24 samples of O-cell agglutination,7 were identified as normal blood group,14 contained MNSs-anti-M antibodies,2 contained cold autoantibodies,and 1 contained warm autoantibodies.Conclusion PK7300 automatic blood grouping analyzer can determine a certain number of ABO subtypes and MNSs-anti-M antibodies,and has the features of simple operation,high automation,high accuracy,and less reagent consuming,which can improve the efficiency and quality of blood group identification,and is suitable for large-scale blood group screening.