基于dsDNA模板的荧光铜纳米簇探针用于药物中乙酰半胱氨酸的无标记快速检测

The rapid and lable-free detection of n-acetylcysteine in pharmaceutical sample based on double-strand DNA-templated fluorescence copper nanoclusters probe

以乙酰半胱氨酸为研究对象,以双链DNA为模板合成的铜纳米簇为荧光探针,建立了一种无标记荧光快速检测方法,并应用于药物的检测。研究发现,乙酰半胱氨酸与铜纳米簇之间可形成Cu-S金属配位键,随着乙酰半胱氨酸浓度的增加,铜纳米簇的荧光强度能够被有效地淬灭。在优化的条件下,方法对乙酰半胱氨酸检测的线性范围为0.2~2.5μmol/L,检测限为42.0 nmol/L,当其他分析物浓度高出10倍时,荧光强度几乎没有变化。该方法在药物样品中对乙酰半胱氨酸的检测,不需要任何荧光染料标记或复杂的DNA序列设计,检测过程均在室温下10 min内完成,加标回收率为99.0%~100.7%。方法适用于低浓度范围内乙酰半胱氨酸的检测。

A novel and label-free fluorescence strategy has been developed for N-acetylcysteine(NAC)detection in pharmaceutical sample by using double-strand DNA-templated synthesis of copper nanoclusters(Cu NCs)as fluorescent probes.Due to the formation of a coordination complex by the Cu-S metal-ligand bond between NAC and double-strand DNA-templated Cu NCs,the fluorescence intensity of double-strand DNA-templated Cu NCs was found to be quenched effectively with the increasing concentration of NAC.Under optimized conditions,the labelfree fluorescent strategy achieved sensitive and selective detection of NAC with a linear range of 0.2-2.5μmol/L and a detection limit of 42.0 nmol/L,and the fluorescence intensity was not changed in the presence of other analytes at a 10-fold higher concentration.Furthermore,this method was successfully applied to the detection of NAC in pharmaceutical sample with good recoveries,which was free of any fluorescence dye label or complex DNA sequence design,and all the processes were performed within 10 min at room temperature.The recoveries ranged from 99.0%to 100.7%.The method is suitable for the determination of acetylcysteine at low concentration.