转氨酶固定化及其连续流催化合成(R)-1-(4-溴苯基)乙胺

Immobilization ofω-transaminase and its catalytic synthesis of(R)-1-(4-bromophenyl)ethylamine in continuous flow

构建连续流反应系统以不对称合成(R)-1-(4-溴苯基)乙胺。通过优化转氨酶ATA-117固定于环氧基树脂载体ES-103B的固定化条件,确定固定化酶ATA117@ES103B的最适反应条件,并对所建连续流反应器系统的工艺条件进行优化。20 mmol/L对溴苯乙酮在7 min的停留时间内转化率即可达到93%,时空产率为789.85 g/(L·d)。研究为生物法催化生产(R)-1-(4-溴苯基)乙胺工业化提供可靠的参考。

It was constructed for a continuous flow reaction system to synthesize(R)-1-(4-bromophenyl)ethylamine asymmetrically.By optimizing the immobilization conditions of transaminase ATA-117 immobilized on the epoxy resin carrier ES-103B,the optimal reaction conditions of immobilized enzyme ATA117@ES103B were determined,and the process conditions of the continuous flow reactor system were optimized.In the constructed continuous flow reaction system,the conversion rate of 20 mmol/L p-bromoacetophenone can reach 93%in 7 min contact time,and the calculated space-time yield is 789.85 g/(L·d).This method provides a reliable reference for the production of(R)-1-(4-bromophenyl)ethylamine by industrialized bio-catalysis.