流感病毒神经氨酸酶及其抑制抗体检测方法的研究进展

Research progress of influenza virus neuraminidase and its inhibitory antibody detection methods

流感病毒表面含有丰富的神经氨酸酶(neuraminidase,NA),其以多种方式参与病毒复制,并促进病毒颗粒从受感染的细胞中释放和传播。NA与流感疫苗的免疫效果有关,NA抑制(NA inhibition,NI)抗体在免疫应答中也起到重要作用。由于目前生产工艺、疫苗类型等原因,疫苗中的NA含量/活性存在差异,如何准确检测疫苗中的NA含量/活性是现在亟待解决的问题。相关检测方法的开发有利于更加全面分析流感疫苗的有效成分,为评估流感疫苗保护效果提供更多的手段。此文就最新的流感病毒NA含量/活性和NI抗体的检测方法进行综述。

Influenza viruses contain large amount of neuraminidase(NA)on their surfaces,which is involved in viral replication as well as release and transmission of viral particles from infected cells in many ways.NA is related to the immune effects of influenza vaccine,and NA inhibition(NI)antibody plays a key role in immune response.At present,the NA content/activity in vaccines is various because of production technology and vaccine type,etc.,so an accurate detection method of NA content/activity in vaccine is in need.The development of relevant detection methods is helpful to analyze the effective components of influenza vaccine and provide more means for evaluating the protective effect of influenza vaccine.This article reviews the latest detection methods of influenza virus NA content/activity and NI antibody.