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自动显微注射过程中图像分割的实现 被引量:1

Realization of image segmentation in automatic microinjection
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摘要 自动显微细胞注射能克服人工注射时间长,易疲劳的缺点,为解决卵胞浆内自动单精子注射过程中细胞和针尖定位问题,通过基于YOLOv3神经网络和水平集相结合的方法,分割卵细胞和注射针,进而定位注射针的针尖。对比实验证明了方法能够解决传统水平集难以分割细胞和注射针的问题,完整分割出显微注射过程中发生形变的细胞,以及实现细胞内针尖的定位,且误差较其他算法小。 Automatic microcell injection can overcome the shortcomings of long manual injection and easy fatigue, in order to solve the problem of cell and pinpoint location in automatic intracytoplasmic sperm injection. Based on the method of combining YOLOv3 neural network and level set, the egg cell and the injection needle are divided, and then the needle tip of the injection needle is located. The comparative experiment proved that the method can solve the problem that the traditional level set is difficult to segment cells and injection needles, and completely segment the deformed cells during microinjection as well as locate the needle tip in the cells, and the error is smaller than other algorithms.
作者 陈冰洁 叶萍 孙京文 吕文尔 杨溪 陈俊强 石盼 常兆华 CHEN Bingjie;YE Ping;SUN Jingwen;LV Wener;YANG XiCHEN Junqiang;SHI Pan;CHANG Zhaohua(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;MicroPort Scientific Corporation,Shanghai 200120,China;Tongji Zhejiang College,Jiaxing 314051,China)
出处 《光学技术》 CAS CSCD 北大核心 2021年第3期379-384,共6页 Optical Technique
关键词 图像分割 注射针 显微注射 神经网络 定位问题 注射时间 显微细胞 针尖 intracytoplasmic sperm injection microinjection image segmentation level set methods YOLOv3
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