结直肠癌组织中HSP90α蛋白表达与伊立替康敏感性的关系分析

Relationship between HSP90αprotein expression and sensitivity of irinotecan in colorectal cancer

目的:检测结直肠癌组织热休克蛋白90α(HSP90α)的表达,并分析其与伊立替康敏感性的关系。方法:对98例结直肠癌手术后复发或转移采用伊立替康化疗患者的资料进行回顾分析,均对手术切除组织采用免疫组化法检测癌组织和癌旁组织HSP90α蛋白表达。对比癌组织和癌旁组织HSP90α蛋白阳性表达率;对比化疗敏感和耐药患者癌组织HSP90α蛋白阳性表达率;采用Logistic回归分析法探讨结直肠癌组织HSP90α蛋白阳性表达与伊立替康化疗敏感的关系。结果:癌组织HSP90α蛋白阳性表达率高于癌旁组织,且差异有统计学意义(P=0.000);伊立替康化疗敏感率为72.45%,耐药率为27.55%,伊立替康化疗耐药患者癌组织HSP90α蛋白阳性表达率高于敏感患者,且差异有统计学意义(P=0.030);Logistic多元回归分析显示,Ⅲ期、癌组织HSP90α蛋白阳性表达均可降低结直肠癌患者伊立替康化疗敏感率。结论:结直肠癌组织HSP90α蛋白阳性表达率高于癌旁组织,且伊立替康化疗耐药患者癌组织HSP90α蛋白阳性表达率更高,Ⅲ期、癌组织HSP90α蛋白阳性表达与伊立替康化疗敏感性均密切相关。

Objective:To detect the expression of heat shock protein 90α(HSP90α) in colorectal cancer and to analyze on its relationship with the chemosensitivity of irinotecan.Methods:The data of 98 patients with postoperative recurrence or metastasis of colorectal cancer treated with irinotecan were retrospectively analyzed.The expressions of HSP90α protein in tumor tissues and adjacent tissues were detected by immunohistochemistry.All patients received irinotecan chemotherapy after recurrence and metastasis.The positive expression rates of HSP90α protein in cancer and adjacent tissues were compared, and the positive expression rates of HSP90α protein in cancer tissues between chemotherapy sensitive and resistant patients were compared.The relationship between the expression of HSP90α protein and chemotherapy sensitivity of irinotecan in colorectal cancer by Logistic regression analysis was determined.Results:The positive expression rate of HSP90α protein in cancer tissues was higher than that in adjacent tissues, and the difference was statistically significant(P=0.000).The sensitivity rate of irinotecan was 72.45%,and the resistance rate was 27.55%.The positive expression rate of HSP90α protein in the cancer tissues of irinotecan resistant patients was higher than that of the sensitive patients, and the difference was statistically significant(P=0.030).Logistic multiple regression analysis showed that stage Ⅲ,HSP90α protein positive expression in cancer tissues can reduce the chemotherapy sensitivity of irinotecan in patients with colorectal cancer.Conclusion:The positive expression rate of HSP90α protein in colorectal cancer is higher than that in the adjacent tissues, and the positive expression rate of HSP90α protein in the cancer tissues of patients with irinotecan resistance is higher.Stage Ⅲ,HSP90α protein positive expression in cancer tissues are closely related to the chemotherapy sensitivity of irinotecan.