藏鸡与白羽肉鸡垂体转录组差异表达研究

Differential Characterization of the Pituitary Transcriptome in Tibetan Chicken and White-feather Broiler

试验旨在通过对藏鸡和白羽肉鸡垂体转录组测序和生物信息学分析,筛选出与鸡生长发育相关的差异表达基因(differentially expression genes,DEGs)及信号通路。本研究选用42日龄健康藏鸡和白羽肉鸡各3只,分别采集垂体组织利用Illumina HiSeq 2000平台进行转录组mRNA测序,对筛选到的DEGs筛选DEGs,GO和KEGG数据库功能注释和富集分析,实时荧光定量PCR验证随机挑选的DEGs表达水平。结果显示,藏鸡和白羽肉鸡分别获得126094302和125666442条clean reads。与白羽肉鸡相比,藏鸡垂体组织中共有DEGs 392个,其中196个为上调基因,196个为下调基因(P<0.05),其中包括生长激素(GH)基因、生长激素释放激素受体(GHRHR)基因和胰岛素样生长因子2 mRNA结合蛋白1(IGF2BP1)基因。GO和KEGG富集分析发现,DEGs显著富集于细胞黏附分子信号通路和神经活性配体-受体相互作用信号通路等细胞通讯相关的信号通路,GH、GHRHR和IGF2BP1基因也显著富集于神经活性配体-受体相互作用信号通路。实时荧光定量PCR结果显示,转录组测序结果准确可靠。综上研究,本研究初步揭示了影响藏鸡和白羽肉鸡生长发育速度差异的关键候选基因和信号通路,为了解鸡垂体组织调节生长发育的分子机制提供了理论依据。

The purpose of this study was to explore the differentially expressed genes(DEGs)and signaling pathways related to growth and development of chicken in pituitary by transcriptome sequencing and bioinformatics analysis.Three 42-day-old healthy Tibetan chickens and White-feather broilers were selected,respectively.The pituitary transcriptome of the 2 chicken breeds was analyzed by Illumina HiSeq 2000 sequencing system.The DEGs were annotated and enriched by GO and KEGG database.The relative expression of randomly selected DEGs were verified by Real-time quantitative PCR.The results showed that 126094302 and 125666442 clean reads were obtained from Tibetan chicken and White-feather broiler,respectively.Compared with White-feather broilers,there were 392 DEGs in Tibetan chickens pituitary,of which 196 up-regulated genes and 196 down-regulated gene(P<0.05),including growth hormone gene(GH),growth hormone releasing hormone receptor gene(GHRHR)and insulin-like growth factor 2 mRNA binding protein 1 gene(IGF2BP1).GO and KEGG enrichment analysis showed that the DEGs were mainly involved in the pathways related to cell communication,such as cell adhesion molecules and neuroactive ligand-receptor interaction signaling pathways.GH,GHRHR and IGF2BP1 genes were also significantly enriched in neuroactive ligand-receptor interaction signaling pathway.The results of Real-time quantitative PCR showed that the accuracy and reliability of the transcriptome sequencing.The research revealed the key candidate genes and signal pathways for the difference in growth and development between Tibetan chicken and White-feather broiler,and provided a theoretical basis for further understanding of the regulatory mechanisms for growth and development of pituitary.