生地黄与马兜铃配伍缓解马兜铃毒性的研究

Study on Compatibility of Rehmannia glutinosa and Aristolochia to Alleviate the Toxicity of Aristolochia

试验旨在探究生地黄与马兜铃配伍对鸡肝脏和肾脏的影响,为中药配伍解毒提供现代理论依据。将80只雄性雏鸡随机分为10组,分别为对照组,马兜铃高、中、低剂量组,生地黄高、中、低剂量组,马兜铃与生地黄配伍高、中、低剂量组。各组中药经过煎煮、过滤、离心去除杂质,制成煎液。每日灌服给药,连续28 d。采用试剂盒检测谷草转氨酶(AST)、谷丙转氨酶(ALT)、肌酐(Cr)、尿素氮(BUN)肝脏和肾脏功能指标;利用试剂盒检测总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)。采集肝脏和肾脏并制备切片观察组织病理学变化。结果显示,与单用马兜铃组相比,马兜铃与生地黄配伍后机体的AST、ALT、Cr和BUN含量显著降低(P<0.05),马兜铃与生地黄配伍后能明显提高机体的T-AOC、SOD和GSH-Px含量;单用马兜铃能引起机体氧化损伤,导致MDA含量显著升高,生地黄与马兜铃配伍后能显著降低MDA含量(P<0.05),减缓马兜铃对机体的氧化损伤;病理结果表明,生地黄与马兜铃配伍后能显著降低马兜铃煎煮液对肝脏和肾脏造成的病理性损伤。综上,与单用马兜铃相比,马兜铃与生地黄配伍联用对鸡肝脏和肾脏造成的损伤有缓解作用,可提高马兜铃的用药安全。

This study was aimed to explore the effect of compatibility of Rehmannia glutinosa and Aristolochia on the liver and kidney of chickens,and provide modern theoretical basis for the compatibility and detoxification of traditional Chinese medicine.80 male chickens were randomly divided into 10 groups:Control group,Aristolochia high,medium and low dose groups,Rehmannia glutinosa high,medium and low dose groups,Aristolochia with Rehmannia glutinosa high,medium and low dose groups.After decocting,filtering and centrifuging,the impurities were removed and the decoction was made.The drug was given by gavage every day for 28 days.The liver and kidney indexes of AST,ALT,Cr and BUN,and T-AOC,SOD,GSH-Px and MDA were detected by kit.The liver and kidney were collected and sectioned to observe the histopathological changes.The results showed that compared with Aristolochia group,the contents of AST,ALT,Cr and BUN were significantly decreased(P<0.05),and the contents of T-AOC,SOD and GSH-Px were significantly increased after compatibility of Rehmannia glutinosa.The combination of Rehmannia glutinosa and Aristolochia could significantly reduce the content of MDA(P<0.05),and slow down the oxidative damage of Aristolochia.The pathological results showed that the compatibility of Rehmannia glutinosa and Aristolochia could significantly reduce the pathological damage of liver and kidney caused by Aristolochia decoction.In conclusion,the combination of Aristolochia and Rehmannia glutinosa could alleviate the liver and kidney injury in mice,and improve the drug safety of Aristolochia.