水貂源铜绿假单胞菌的分离鉴定、耐药性及毒力基因检测

Isolation,Identification,Drug Resistance and Virulence Genes Detection of Pseudomonas aeruginosa from Mink

为查明河北某水貂养殖场水貂死亡原因,本研究进行了病理组织学检查、细菌分离培养、细菌形态学观察、生化鉴定、16S rDNA鉴定、药敏试验、耐药基因和毒力基因检测、动物回归试验等。结果显示,共分离到3株铜绿假单胞菌,16S rDNA序列与GenBank中铜绿假单胞菌相应序列相似性达到100%,且3株分离菌均能使大鼠死亡。药敏结果表明,分离菌对左氧氟沙星、庆大霉素、阿米卡星、多黏菌素B敏感,对β-内酰胺类、氨基糖苷类、大环内酯类、喹诺酮类、四环素类等抗生素耐药。分离菌携带3种超广谱β-内酰胺酶基因(bla_(CTX-M1)、bla_(OXA-2)、bla_(OXA-10))、3种碳青霉烯酶基因(bla_(VIM-1)、bla_(SPM-1)、bla_(KPC-1))及吸附相关基因、T1SS-T3SS分泌系统、氧化应激相关基因、群体行为调控基因、磷脂酶相关的17种毒力基因。本研究结果为水貂绿脓杆菌病的临床诊断和治疗提供了参考依据。

To find out the cause of mink death in Hebei,pathogen identification was conducted by histopathological examination,bacterial isolation and culture,morphological observation,biochemical identification,drug susceptibility test,drug resistance gene and virulence gene detection and animal regression experiment.The results showed that 3 strains of Pseudomonas aeruginosa were isolated,3 strains had 100%similarity with strains of Pseudomonas aeruginosa in GenBank,and all 3 strains could cause the death of the rats.Drug susceptibility test results showed that the isolates were highly susceptible to levofloxacin,gentamycin,amikacin,polymyxin B and ceftriaxone,and were resistant to extended-spectrumβ-lactamases(ESBLs),aminoglycosides,macrolides,quinolones and tetracyclines.The isolates carried ESBLs genes(bla_(CTX-M1),bla_(OXA-2),bla_(OXA-10)),carbapenemases(bla_(VIM-1),bla_(SPM-1),bla_(KPC-1)),and 17 virulence-related markers including adhesion,T1SS-T3SS,oxidativestress,quorum sensing and regulation,and phospholipids.The results would provide references for disease control,therapeutical guidance and drug administration in mink.