基于纳米磁流体尸体CT血管造影的可行性探究

Research on the feasibility of applying magnetic nanoparticle(MNP)ferrofluid to postmortem CT angiography(PMCTA)

目的初步探索纳米磁流体(magnetic nanoparticle ferrofluid,MNPF)用于尸体计算机断层扫描血管造影(postmortem computed tomography angiography,PMCTA)的可行性。方法纳米粒子γ-Fe2O3(30nm)通过油酸(oleic acid,OA)进行表面修饰,修饰后物质移至液体石蜡油(paraffin oil,PO)中,使用高速搅拌器搅拌,制备成γ-Fe2O3含量为200g/L的MNPF。使用手动负压灌注法,将MNPF、PO和OA分别导入3只牛心脏的冠状动脉及左心室。将灌有不同液体的牛心移至CT机上扫描,观察冠脉或左心室显影情况。结果灌注MNPF的牛心,其冠脉呈现高信号,3D重建后可以较好地显示冠脉解剖形态。灌注PO和OA的牛心,冠脉呈现低信号,无法通过3D重建显示冠脉解剖形态。结论MNPF可以提高冠脉显影效果,对于PMCTA可能有一定的研究价值。

Objective To study the feasibility of applying magnetic nanoparticle ferrofluid(MNPF)to postmortem computed tomography angiography(PMCTA).Methods Surface of nanoparticleγ-Fe2O3(30 nm)was modified by oleic acid(OA).The modified material was transferred into paraffin oil(PO)and mixed by a high power mixer to prepare MNPF.MNPF,PO and OA were introduced into corontary artery and left vetricle of three bovine hearts by manual negativepressure perfusion method.Results A hyperintensity of coronary artery was observed in the bovine heart perfused with MNPF,and the radiological morphology of its coronary artery could be visualized on 3 D reconstruction images.On the contrary,hypointensities were observed in the bovine hearts perfused with PO and OA,and the coronary arteries were not visualized on 3 D reconstruction images.Conclusion MNPF can enhance the contrast of coronary artery,which may have certain research value for PMCTA.