生物酶法改性玉米皮渣膳食纤维工艺研究

Bio-enzymatic Modification for Dietary Fiber of Corn Bran

该研究以玉米皮渣为原料,经处理后获得不溶性膳食纤维,采用生物酶法对膳食纤维进行改性处理,主要研究双酶酶解工艺对水溶性膳食纤维得率的影响。结果表明,木聚糖酶和纤维素酶酶解玉米皮渣可显著提高可溶性膳食纤维得率,最佳的酶解条件为纤维素酶添加量为30 mg/g底物、木聚糖酶添加40 mg/g底物、料液比1∶12(g/mL)、酶解时间90 min,在该条件下,水溶性膳食纤维得率为2.996%。

In this study,corn bran residue was used as raw material to obtain insoluble dietary fiber after treatment.The dietary fiber was modified by biological enzyme method.The effect of double enzyme hydrolysis process on the yield of water-soluble dietary fiber was mainly studied.The results showed that xylanase and cellulase hydrolysis of corn bran residue could significantly improve the yield of soluble dietary fiber.The optimal hydrolysis conditions were as follows:cellulase 30 mg/g substrate,xylanase 40 mg/g substrate,solidliquid ratio 1∶12(g/mL),hydrolysis time 90 min.Under these conditions,the yield of soluble dietary fiber was 2.996%.