circ_0072088通过调控miR-545-3p/STAT3轴促进非小细胞肺癌细胞的恶性生物学行为

circ_0072088 promotes the malignant biological behaviors of non-small cell lung cancer cells by regulating miR-545-3p/STAT3 axis

目的:探究环状RNA(circular RNA,circRNA)0072088在非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞中的生物学功能及其作用机制。方法:在公共基因芯片数据库Gene Expression Omnibus(GEO)中下载GSE101684数据集,通过GEO2R分析得到差异基因。通过qPCR检测NSCLC细胞H165、H358、H460、H226和A549细胞中circ0072088的表达水平,随后采用CCK-8法和Transwell小室法分别检测circ0072088对NSCLC细胞增殖、迁移和侵袭的作用。通过CircInteractome和TargetScan数据库预测circ0072088与miR-545-3p、miR-545-3p与STAT3之间的靶向关系,并通过双荧光素酶报告基因实验和RNA结合蛋白免疫沉淀(RNA binding protein immunoprecipitation,RIP)实验验证circ0072088、miR-545-3p与STAT3之间的靶向关系。结果:circ0072088在NSCLC细胞系中的表达均显著上调(均P<0.05)。过表达circ0072088促进了NSCLC细胞的增殖、侵袭和迁移(均P<0.05);敲低circ0072088抑制了NSCLC细胞的增殖、侵袭和迁移(均P<0.05)。miR-545-3p是circ0072088的下游靶点,可以被circ0072088吸附;STAT3是miR-545-3p的靶基因,可以被circ0072088间接正向调控。结论:Circ0072088通过调节miR-545-3p/STAT3轴促进NSCLC细胞的增殖和转移。

Objective:To explore the biological function and mechanism of circular RNA(circRNA)0072088 in non-small cell lung cancer(NSCLC)cells.Method:GSE101684 Gene expression profilesdata set were was downloaded from Gene Expression Omnibus(GEO,GSE101684),and the differentially expressed genes(DEGs)were screened with GEO2 R analysis was used to obtain differential genes.qPCR was used to detect tThe expression of circ0072088 in NSCLC cells cell lines(H165,H358,H460,H226 and A549 cells)was detected using qPCR.CCK-8 assay and Transwell cell methodchamber assays were utilized to assess cell proliferation,migration and invasion.CircInteractome and TargetScan database,dual luciferase reporter gene experiment and RNA binding protein immunoprecipitation(RIP)experiment were were used to predict and verify the targeting relationship between circ0072088 and miR-545-3 p,as well as between miR-545-3 p and STAT3,which were then verified by Dual luciferase reporter gene experiment and RNA binding protein immunoprecipitation(RIP)experiment.Results:The expression of circ0072088 in NSCLC cell lines was significantly up-regulated(P<0.05).Over-expression of circ0072088 promoted the proliferation,invasion and migration of NSCLC cells(P<0.05);knock down of circ 0072088 inhibited the proliferation,invasion and migration of NSCLC cells(P<0.05).MiR-545-3 p is the downstream target of circ0072088 and can be sponged by circ0072088;STAT3 is the target gene of miR-545-3 p,which can be indirectly and positively regulated by circ0072088.Conclusion:Circ0072088 promotes the proliferation and metastasis of NSCLC cells by regulating the miR-545-3 p/STAT3 axis.