血清miR-34a水平与阿尔茨海默病及认知功能障碍的相关性

Correlation between serum miR-34a level and Alzheimer's disease and cognitive dysfunction

目的探讨阿尔茨海默病(AD)患者血清miR-34a水平的意义及其与认知功能障碍的相关性。方法收集2018年10月至2020年1月于南阳市第二人民医院就诊的老年AD患者和轻度认知功能障碍(MCI)患者各60例,纳入同期体检健康老年人60例为对照组。采用定量反转录酶-聚合酶连锁反应(qRT-PCR)检测三组受试者血清miR-34a水平;检测其他实验室指标LDL-C、HDL-C、Hcy、TG和TC的水平,采用Pearson相关系数、Logistic回归和ROC曲线分析miR-34a和AD患者认知障碍的关系。结果AD组和MCI组患者血清中miR-34a显著高于对照组,且AD组高于MCI组(P<0.05)。miR-34a与Hcy、TG和TC水平成正相关,与MMSE和MoCA评分成负相关。miR-34a是认知功能障碍及MCI进展为AD的独立影响因素,其诊断认知功能障碍及鉴别MCI和AD的曲线下面积分别为0.967和0.765。结论miR-34a在AD患者血清中高表达,且与认知功能成负相关,对于认知功能障碍和AD具有一定的诊断价值。

Objective To explore the significance of serum miR-34a level in patients with Alzheimer's disease and cognitive dysfunction.Methods 60 elderly AD patients and 60 mild cognitive impairment(MCI)patients who visited Nanyang Second Genera Hospital from October 2018 to January 2020 were collected,60 healthy elderly patients who took medical examination in the same period were collected as control group.Quantitative reverse transcriptase-polymerase chain reaction(qRT-PCR)was used to detect the serum miR-34a levels of the three groups;Laboratory indicators LDL-C,HDL-C,Hcy,TG and TC were also detected;Pearson correlation coefficient,Logistic regression and ROC curve were used to analyze the relationship between miR-34a and cognitive impairment in patients with AD.Results The serum miR-34a in AD group and MCI group were significantly higher than the control group,and that in the AD group was higher than the MCI group(P<0.05).miR-34a is positively correlated with Hcy,TG and TC levels,and negatively correlated with MMSE and MoCA scores.miR-34a is an independent factor influencing cognitive dysfunction and the progression of MCI into AD.The area under the curve for diagnosing cognitive dysfunction and distinguishing MCI from AD is 0.967 and 0.765,respectively.Conclusion miR-34a is highly expressed in the serum of AD patients and negatively correlated with cognitive function,which has certain diagnostic value for cognitive dysfunction and AD.