黄骅特产皮皮虾虾壳中甲壳素的制备研究

Study on Chitin Preparation from Shrimp Shells in Huanghua

甲壳素应用广泛,水产品废弃物往往用作甲壳素的主要制备原料。该研究采用中性蛋白酶去除蛋白质,结合醋酸脱钙的方法处理黄骅特产皮皮虾虾壳,利用单因素和响应面试验对从黄骅特产皮皮虾中制备甲壳素的工艺进行优化。结果表明,在脱蛋白质步骤中,处理时间2 h、温度48℃、酶添加量5000 U为最佳工艺条件。在脱钙步骤中,处理时间1 h、温度20℃、水与醋酸体积比9∶1为最佳工艺条件。经过本研究优化后的甲壳素最终得率为6.87%。

Chitin is widely used,and waste from aquatic products is often the main source of raw material for chitin preparation.In this study,shrimp shells in Huanghua were used as the source material,and chitin preparation technology was optimized using single factor and response surface experiments combining deproteinization with neutral protease,and decalcification using acetic acid.The best conditions for deproteinization were 2 h at 48℃with 5000 U of neutral protease.The best conditions for decalcification were 1 h at 20℃,and the best technological conditions of decalcification was the ratio of water to acetic acid was 9∶1.Using these optimized conditions,chitin yield was 6.87%.