摘要
目的探究人工繁育的恒河猴群体中肺炎链球菌的自然感染状况,为肺炎链球菌感染的临床诊断和治疗提供参考。方法从恒河猴猴群中随机采集20只婴猴、30只成年猴和30只老年猴的鼻拭子和咽拭子样本,采用细菌培养和实时荧光定量PCR方法同时筛查样本中肺炎链球菌的携带情况,并对筛查结果为阳性的婴猴的亲代母猴进行肺炎链球菌筛查。再结合革兰染色法、奥普托欣药敏试验、胆汁溶解试验、菊糖发酵试验和PCR扩增测序对样本进行进一步鉴定。结果恒河猴婴猴携带肺炎链球菌的检出率为20%(4/20),而这4只阳性婴猴的亲代母猴检测结果均为阴性。成年恒河猴和老年恒河猴中肺炎链球菌的检出率均为0%。细菌培养物在形态学上观测显示,该菌为灰白色凸起状,周围草绿色溶血环,呈脐窝状,为典型肺炎链球菌的菌落特征。革兰染色结果显示为阳性,胆汁溶解试验和菊糖发酵试验结果均呈阳性,并且该菌对奥普托欣敏感;以上结果表明本次从婴猴鼻咽拭子分离到的4株菌落均为肺炎链球菌。结论本调查发现在恒河猴中肺炎链球菌自然感染群体主要为婴猴,婴猴携带率约为20%,其鼻、咽部均为该菌寄住场所,且未发现母婴垂直传播情况。
Objective To explore the natural infection status of Streptococcus pneumoniae in captive bred rhesus macaques,and to provide reference for the clinical diagnosis and treatment of Streptococcus pneumoniae infection.Methods Twenty infant monkeys,30 adult monkeys and 30 elderly monkeys were randomly selected from a rhesus macaques population,and the nasal swabs and pharynx swabs were collected and screened for Streptococcus pneumoniae simultaneously by bacterial culture and real-time fluorescent quantitative PCR.The mother monkeys of the positive infant monkeys were screened for Streptococcus pneumoniae.Gram staining method,Optochin drug sensitivity test,bile solubility test,inulin fermentation test and PCR amplification and sequencing were used to further identification.Results The positive rate of rhesus macaques carrying Streptococcus pneumoniae was 20%(4/20),and the mother macaques of the four positive infant macaques tested negative.The positive rate was 0%in both adult and elderly rhesus monkeys.The morphological observation results of the bacterial culture showed that the bacteria appeared gray and raised,and produced a surrounding green zone of hemolysis with a flattened center,indicating typical Streptococcus pneumoniae colony characteristics.The results of Gram dyeing,bile dissolution test,and inulin fermentation test were all positive,and the bacteria were sensitive to Optochin.The above results showed that the four strains isolated from the infant monkeys’nasopharyngeal swabs were Streptococcus pneumoniae.Conclusion The investigation findings show that Streptococcus pneumoniae is mainly found in the noses and throats of 20%infant monkeys among rhesus macaques,and no vertical transmission from mother to child is found.
作者
刘雨
李艳艳
张伟
杨凤梅
刘权
李咏洁
靳玮华
段素琴
王俊斌
陈丽雄
徐鸿界
赵远
和占龙
LIU Yu;LI Yanyan;ZHANG Wei;YANG Fengmei;LIU Quan;LI Yongjie;JIN Weihua;DUAN Suqin;WANG Junbin;CHEN Lixiong;XU Hongjie;ZHAO Yuan;HE Zhanlong(Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases,Kunming 650118,China)
出处
《实验动物与比较医学》
CAS
2021年第3期259-265,共7页
Laboratory Animal and Comparative Medicine
基金
中国医学科学院医学与健康科技创新工程项目(2016-I2M-2-001,2018-I2M-3-002)
云南省科技创新人才计划(2015HC027)
昆明市科技创新和服务能力提升计划重点项目(2016-2-R-07674)。
关键词
恒河猴
肺炎链球菌
携带率
婴猴
Rhesus macaques
Streptococcus pneumoniae
Carrying rate
Infant monkeys