电针“神门”对急性心肌缺血模型大鼠心肌和海马组织以及血清中cAMP和cGMP表达的影响

Effect of electroacupuncture"Shenmen"on the expression of cAMP and cGMP in the myocardium,hippocampus and serum of rats with acute myocardial ischemia

目的:从环磷酸腺苷(cyclic adenosine monophosphate,cAMP)和环磷酸鸟苷(cyclic guanosine monophosphate,cGMP)表达的角度,探讨电针“神门”对大鼠急性心肌缺血模型所致心脑损伤的保护作用。方法:将30只雄性SD大鼠随机分为正常组、模型组、电针组,Powerlab 8导生理记录系统记录大鼠心电图,行冠状动脉左前降支结扎法复制大鼠心肌缺血模型,电针组在模型复制后第2天予以电针治疗,末次电针治疗后采集大鼠心肌和海马组织以及腹主动脉血,采用酶联免疫吸附法检测心肌、海马组织和血清中cAMP和cGMP的水平。结果:与正常组比较,模型组大鼠心肌组织中cAMP水平明显升高,海马组织和血清中cAMP的水平明显降低,差异有统计学意义(P<0.05);与模型组比较,电针组大鼠心肌组织中cAMP水平降低,海马组织和血清中cAMP的水平升高,差异有统计学意义(P<0.05);与正常组比较,模型组大鼠心肌组织和血清中cGMP的水平升高,海马组织中cGMP的水平降低,差异有统计学意义(P<0.05);与模型组比较,电针组大鼠海马组织中cGMP水平升高,血清中cGMP的水平明显降低,差异有统计学意义(P<0.05),心肌组织中cGMP水平的差异无统计学意义(P<0.05)。结论:电针“神门”能显著改善急性心肌缺血致心脑损伤的模型大鼠血清和海马的cAMP、cGMP以及心肌cAMP的表达,但对心肌cGMP的影响较小。

Objective:To discuss the protection of electroacupuncture "Shenmen" on heart and brain injury induced by acute myocardial ischemia in rats from the perspective of the expression of cyclic adenosine monophosphate(cAMP)and cyclic guanosine monophosphate(cGMP). Methods:Thirty male SD rats were randomly divided into the normal group,the model group,and the electroacupuncture group. The electrocardiogram was recorded by the Powerlab 8-lead physiological recording system. The left anterior descending coronary artery was ligated to replicate the rat myocardial ischemia model. The acupuncture group was treated with electroacupuncture on the second day after the model was replicated. After the last electroacupuncture treatment,rat myocardium,hippocampus tissue and abdominal aortic blood were collected,and enzyme-linked immunosorbent assay was used to detect the levels of cAMP and cGMP in myocardium,hippocampus tissue and serum content(P<0.05). Results:Compared with the normal group,the cAMP content in the myocardial tissue of the model group was significantly increased,and the cAMP content in the hippocampus tissue and serum was significantly reduced;compared with the model group,the cAMP content in the myocardial tissue of the electroacupuncture group was decreased(P<0.05),and in the hippocampus tissue and serum was significantly increased. Compared with the normal group,the content of cGMP in the myocardial tissue and serum of the model group was increased(P<0.05),and the content of cGMP in the hippocampus was decreased(P<0.05). Compared with the model group,the content of cGMP in the hippocampus of the electroacupuncture group was increased(P<0.05). The cGMP was content increased and the serum cGMP content was significantly reduced(P<0.05),and the difference in the cGMP content in myocardial tissue was not statistically significant(P<0.05). Conclusion:Electroacupuncture at "Shenmen" acupoint can significantly improve the expression of cAMP,cGMP and myocardial cAMP in the serum and hippocampus of model rats with acute myocardial ischemia-induced heart and brain injury,but has a lower effect on myocardial cGMP content.