青藤碱星状神经节阻滞对慢性心肌缺血大鼠的影响

Effect of sinomenine stellate ganglion block on chronic myocardial ischemia in rats

探讨青藤碱星状神经节阻滞(SGB)对慢性心肌缺血的保护作用及其相关机制。方法:SD雌雄各半大鼠(180~200 g)40只,随机分为4组,空白组、模型组、利多卡因组、利多卡因+青藤碱组。空白组大鼠饲喂普通标准饲料,不造模,其余大鼠喂高脂饲料。饲喂8周后,高脂饲料喂养大鼠与空白对照组相比,血脂有明显差异之后随机分为3组。模型组给予右侧星状神经节(RSG)注射0.9%NaCl 0.24 mL,连续注射2周后,于大鼠舌下静脉连续3 d注射垂体后叶素注射液,1次/24 h;利多卡因组给予RSG注射1%利多卡因注射液0.24 mL,余同模型组;利多卡因+青藤碱组给予RSG注射1%利多卡因注射液0.24 mL+盐酸青藤碱0.095 mL+0.9%生理盐水2.9 mL,共0.8 mL,余同模型组。实验第8周末高脂饲料喂养组与标准普通饲料喂养大鼠血脂有明显差异后进行给药。第3次注射垂体后叶素前观察各组大鼠心电图变化;观察给药前后大鼠一般情况;实验结束后各组大鼠腹主动脉取血,检测血清氧化应激指标总超氧化物歧化酶(SOD)、丙二醛(MDA)、白介素⁃6(IL⁃6)及肌钙蛋白I(CTnI)水平。结果:与空白组比较,模型组心电图变化明显(P<0.01)、CTnI值明显升高(P<0.01),表明大鼠心肌缺血模型制备成功;与模型组比较,利多卡因组、利多卡因+青藤碱组SOD水平明显上升,MDA水平明显下降、IL⁃6水平明显降低,差异均具有统计学意义(P<0.05)。结论:青藤碱SGB对慢性心肌缺血大鼠有保护作用,其保护作用与抗氧化应激及抑制炎症反应有关。

Objective:To investigate the protective effect of sinomenine stellate ganglion block(SGB)on chronic myocar‐dial ischemia and its related mechanism.Methods:SD male and female rats(180-200 g)were randomly divided into four groups:blank group,model group and lidocaine group,lidocaine+sinomenine group.The rats in the blank group were fed with normal standard diet without modeling,and the other rats were fed with high‐fat diet.After 8 weeks of feeding,the rats in the high‐fat diet group were significantly different from those in the blank control group.Then they were randomly divided into 3 groups and 10 rats in the model group were injected with 0.9%NaCl into right stellate ganglion(RSG).After 2 weeks of continuous injection,pitui‐trin injection was continuously injected into sublingual vein of rats for 3 days,once every 24 hours;lidocaine group rats were inject‐ed with 0.24 mL 1%lidocaine injection in RSG,the rest was the same as model group;The lidocaine+sinomenine group was given RSG injection of 0.24 mL of 1%lidocaine injection+sinomenine hydrochloride 0.095 mL+0.9%saline 2.9 mL,a total of 0.8 mL,the rest was the same as the model group.At the end of the eighth week of the experiment,the rats in the high‐fat diet feeding group and the standard ordinary diet feeding group were given the medicine after there was a significant difference in blood lipid;before the third injection of pituitrin,the ECG changes of the rats in each group were observed;the general situation of the rats before and after the administration was observed;after the experiment,the blood of the rats in each group was taken from the abdominal aorta,and the serum oxidative stress indexes,such as total superoxide dismutase(SOD)and malondialdehyde,were detected(MDA,IL‐6 and cTnI were measured.Results:Compared with the blank group,the ECG of the model group changed significantly(P<0.01),the cTnI value increased significantly(P<0.01),indicating that the rat myocardial ischemia model was successfully established;compared with the model group,the SOD level of lidocaine group and lidocaine+sinomenine group in‐creased significantly,the MDA level decreased significantly,IL‐6 decreased significantly,the differences were statistically signifi‐cant(P<0.05).Conclusion:Sinomenine SGB has a protective effect on rats with chronic myocardial ischemia,which is related to anti oxidative stress and inhibition of inflammatory reaction.