基于网络药理学探讨夏枯草对Graves病的作用机制

Mechanism of Prunella vulgaris in treating Graves'disease based on network pharmacology

目的:运用网络药理学方法筛选夏枯草治疗Graves病的有效成分和作用靶点,探究其成分⁃靶点⁃疾病的关系。方法:通过中药系统药理学平台(TCMSP)收集夏枯草的活性成分及作用靶点;通过GeneCards及DisGeNET数据库收集Graves病的疾病相关基因,构建“药物⁃成分⁃疾病⁃靶点”网络并筛选出夏枯草治疗Graves病的核心靶点。然后进一步进行蛋白互作网络分析、GO生物功能注释、KEGG通路分析及分子对接验证。结果:夏枯草的10个活性成分可通过调控PPARG、PIK3CG、CASP9、AKT1、TNF、ICAM1、BCL2、BAX等57个Graves病的疾病靶点,影响炎症应答、凋亡过程的负调控等相关生物学过程及TNF信号通路、HIF⁃1信号通路、PI3K⁃Akt信号通路及癌症相关通路等相关信号通路,从而发挥治疗Graves病的作用。结论:本研究初步验证了夏枯草治疗Graves病的靶点及作用方式,可为进一步揭示其临床作用机制奠定基础。

Objective:To screen the effective components and targets of Prunella vulgaris to treat Graves’disease using the network pharmacology,and to explore the relationship between its components and targets and diseases.Methods:Active components and targets of Prunella vulgaris were collected and screened through TCMSP;disease‐related genes of Graves’dis‐ease were screened using GeneCards and DisGeNET databases;a"drug‐component‐disease‐target"network was constructed to screen the core target of Prunella vulgaris to treat Graves’disease.Then protein-protein interaction(PPI)network analysis,GO biological function annotation,and KEGG pathway analysis and molecular docking verification were carried out.Results:The ten active components of Prunella vulgaris can control 57 Graves'disease targets such as PPARG,PIK3CG,CASP9,AKT1,TNF,ICAM1,BCL2 and BAX,etc.,affecting the inflammatory response,negative regulation of apoptosis and other related or‐ganisms.Academic process and TNF signaling pathway,HIF‐1 signaling pathway,PI3K‐Akt signaling pathway and cancer‐relat‐ed pathways and other related signaling pathways,thus playing a role in the treatment of Graves’disease.Conclusion:This study initially verified the target and mode of action of Prunella vulgaris for Graves’disease,which can lay the foundation for further re‐vealing its clinical mechanism of action.