电针联合有氧运动对脑梗死后缺血半暗带cAMP/PKA通路的影响

Effect of electroacupuncture combined with aerobic exercise on cAMP/PKA pathway in ischemic penumbra after cerebral infarction

目的:观察电针联合有氧运动对脑梗死大鼠运动功能的影响,并探讨其对cAMP/PKA信号通路的影响。方法:将SD大鼠随机分为空白组、模型组、电针组、有氧运动组、电针+有氧运动组,各18只,电针组于造模24 h后对大鼠进行电针曲池、足三里穴干预;有氧运动组造模24 h后予匀速跑台训练;电针+有氧运动组在电针曲池、足三里穴后再予跑台训练,各组均干预7 d。观察各组大鼠神经行为学、脑梗死体积、神经元细胞超微结构变化,同时检测脑组织cAMP、PKA蛋白表达变化。结果:神经行为学显示电针+有氧运动组可明显下调脑梗死大鼠的神经缺损评分、缩小脑梗死体积、改善缺血半暗带内神经元细胞超微结构损伤、上调cAMP、PKA蛋白水平,与模型组、电针组、有氧运动组比较,差异均具有统计学意义(P<0.05)。结论:电针联合有氧运动可促进脑梗死大鼠运动功能恢复,其作用机制可能与激活cAMP/PKA信号通路有关。

Objective:To observe the effect of electroacupuncture combined with aerobic exercise on motor function in rats with cerebral infarction and explore its effect on cAMP/PKA signal pathway.Methods:SD rats were randomly divided into blank group,model group,EA group,aerobic exercise group and EA+aerobic exercise group,with 18 rats in each grou.The aerobic exercise group received treadmill training 24 hours after modeling.The electroacupuncture+aerobic exercise group received treadmill training after electroacupuncture at Quchi and Zusanli,and each group was intervened for 7 days.The changes of neuroethology,infarct volume and ultrastructure of neurons were observed,and the expressions of AMP and PKA protein in brain tissue were detected.Results:Neuroethology showed that EA+aerobic exercise group could significantly reduce the neurological deficit score,reduce the volume of cerebral infarction,improved the ultrastructural damage of neurons in the ischemic penumbra,and up regulate the levels of camp and PKA protein in rats with cerebral infarction,and the difference was statistically significant compared with model group,EA group and aerobic exercise group(P<0.05).Conclusion:Electroacupuncture combined with aerobic exercise can promote the recovery of motor function in rats with cerebral infarction,and its mechanism may be related to the activation of cAMP/PKA signal pathway.