水曲柳中MYBL2基因表达特征分析

Characteristic analysis of FmMYBL2 gene expression in Fraxinus mandshurica

MYB转录因子家族是植物中最大的转录因子家族之一,参与植物生长、繁殖和代谢的各个时期,能通过多种方式参与植物抗逆生长。该文在水曲柳中克隆FmMYBL2基因,利用生物信息学分析其结构和表达特征,并构建FmMYBL2蛋白的系统进化树。对水曲柳幼苗进行低温胁迫、盐胁迫处理以及激素分子诱导处理(包括ABA、IAA、GA 3、JA、SA)。分别在0、1、3、6、12、24、48 h取样,利用实时荧光定量PCR对上述处理样品中FmMYBL2基因进行定量分析,并分析了FmMYBL2的时空表达特征。结果表明:(1)克隆得到的FmMYBL2基因全长为762 bp,编码253个氨基酸。(2)FmMYBL2蛋白是亲水性蛋白,氨基酸序列比对表明其与棉花同源关系较近。(3)荧光定量分析表明,FmMYBL2基因响应低温胁迫和盐胁迫,同时ABA、IAA、GA 3、JA、SA共同调控该基因表达。(4)在低温处理1 h、盐胁迫48 h时,虽然FmMYBL2基因表达量最高,激素诱导后表达量持续波动,但其能在短时间内迅速响应。(5)FmMYBL2基因在根、芽、花、种子中均有表达,雄花中的表达量最高。该研究结果为深入研究MYBL2基因功能和水曲柳抗逆生长的调控奠定基础。

The MYB transcription factor family is one of the largest transcription factor families in plants and it is involved in all stages of plant growth,reproduction and metabolism,and can participate in plant stress resistance growth in a variety of ways.The FmMYBL2 gene was cloned in Fraxinus mandshurica,and its structure and expression characteristics were analyzed using bioinformatics and a phylogenetic tree of FmMYBL2 was constructed.Low temperature,salt and hormone molecules(including ABA,IAA,GA 3,JA,SA)were used to induce F.mandshurica seedlings.Samples were taken at 0,1,3,6,12,24,48 h.Quantitative analysis of FmMYBL2 gene in the treated samples.The temporal and spatial expression characteristics of FmMYBL2 were analyzed.The results were as follows:(1)The FmMYBL2 gene was 762 bp in length and encodes 253 amino acids.(2)FmMYBL2 encoded a hydrophilic protein.Amino acid sequence alignment showed that it was closely related to Gossypium hirsutum.(3)Quantitative fluorescence analysis showed that FmMYBL2 gene responded to low temperature and salt stress.At the same time,ABA,IAA,GA 3,JA and SA jointly regulated the expression of FmMYBL2 gene.(4)The gene expression was the highest under low temperature treatment for 1 h and salt stress for 48 h.After hormone induction,the expression of this gene fluctuates continuously,but it can respond quickly in a short time.(5)Expression of this gene was observed in roots,buds,flowers and seeds and the male flowers had the highest expression.The results of this study provide reference for further studying the function of MYBL2 gene and the regulation of stress resistance of Fraxinus mandshurica.