高效分子排阻色谱法测定滇黄精多糖的分子量

Determination of Molecular Weight of Polygonatum Kingianum Polysaccharide by HPSEC

目的建立测定滇黄精多糖分子量的高效分子排阻色谱法。方法色谱柱采用TSK G3000 PWXL凝胶柱(300 mm×7.8 mm,10μm),配有Shodex RI-101型示差检测器,检测波长为210 nm,流动相为纯水,流速为0.5 m L/min,柱温为30℃,进样量为20μL。结果葡聚糖对照品重均分子量(Mw)的线性范围为10.0×10^(3)~200×10^(3)。滇黄精多糖组分PK-F1和PK-F2中多糖主峰P1的保留时间(t_(R))为10.55 min,Mw为145850;多糖主峰P2的t_(R)为12.78 min,Mw为24565。滇黄精多糖组分PK-F3多糖主峰P3的t_(R)为16.91 min,Mw为907。结论该方法简易、准确、可靠,可为滇黄精的质量控制提供参考。

Objective To establish a high-performance size exclusion chromatography(HPSEC)method for the determination of the molecular weight of Polygonatum kingianum polysaccharide.Methods The TSK G3000 PWXL gel column(300 mm×7.8 mm,10μm)was adopted with Shodex RI-101 differential detector,the detection wavelength was 210 nm,the mobile phase was pure water,the flow rate was 0.5 m L/min,the column temperature was 30℃,and the injection volume was 20μL.Results The molecular weight linear range of dextran was from 10.0×10^(3) to 200×10^(3).In PK-F1 and PK-F2 Polygonatum kingianum polysaccharide,the retention time(t_(R))of main peak P1 was 10.55 min and the molecular weight was 145850,the t_(R) of main peak P2 was 12.78 min and the molecular weight was 24565.In PK-F3 Polygonatum kingianum polysaccharide,the t_(R) of main peak P3 was 16.91 min and the molecular weight was 907.Conclusion The method is accurate and reliable,which can provide a reference for the quality control of Polygonatum kingianum.