摘要
为从土壤中筛选出纤维素高效降解菌株,本研究以宁夏扬黄灌区的13个土壤样品为研究对象,通过菌种分离和纯化,共获得纯化菌株836株,其中真菌122株,细菌543株,放线菌171株。通过羧甲基纤维素钠培养基结合刚果红染色法进行筛选,以菌落直径与产生水解圈直径比值为评判指标,获得具有较强纤维素降解功能的菌株26株。对这些菌株进行培养特征观察和显微观察,初步鉴定其中放线菌10株,细菌16株。研究结果旨在为进一步开展秸秆纤维素高效降解菌的筛选及应用提供技术支撑。
In order to screen out cellulose-degrading bacteria from soil,13 soil samples were collected from the Yellow River irrigation area in Ningxia,and a total of 836 strains,including 122 fungi,543 bacteria and 171 actinomycetes,were isolated and purified.A total of 26 strains with strong cellulose degradation abilities were screened by using the ratio of colony diameter to the diameter of hydrolytic zone as the evaluation index through the combination of sodium carboxymethyl cellulose medium and Congo red staining.The culture characteristics and microscopic observation of these strains,10 strains of actinomycetes and 16 strains of bacteria were preliminarily identified.The results can provide technical support for further screening and application of straw cellulose-degrading bacteria.
作者
朱金霞
周文生
孔德杰
冯锐
杨佳璇
Zhu Jinxia;Zhou Wensheng;Kong Dejie;Feng Rui;Yang Jiaxuan(Agricultural Biotechnology Research Center,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002;Ningxia Nuclear Industry Geological Prospecting Institute,Yinchuan,Ningxia 750021;Institute of Agricultural Economy and Information Technology,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002)
出处
《宁夏农林科技》
2021年第3期24-27,F0002,共5页
Journal of Ningxia Agriculture and Forestry Science and Technology
基金
宁夏农林科学院农业自主科技创新对外科技合作专项(DWX-2018026、DWX-2020007)
宁夏回族自治区重点研发计划项目(2020BBF03006)
宁夏回族自治区重点研发项目“贺兰山非金属露天矿山生态恢复专用基质研究与示范”。
关键词
纤维素降解菌
生物降解
初筛
形态学观察
Cellulolytic bacteria
Biodegradation
Isolation
Morphological observation
