玉米醇溶蛋白/聚环氧乙烷同轴静电纺丝负载姜黄素及其释放特性

Release Characteristics of Curcumin Loaded on Zein/Poly (Ethylene Oxide) by Coaxial Electrospinning

玉米醇溶蛋白作为生产淀粉的副产物,大多应用在饲料方面,利用率低,本实验借助静电纺丝技术高值化利用玉米醇溶蛋白得到纳米纤维膜,并用于抗菌方面的研究。通过同轴静电纺丝技术研发具有核/壳结构负载姜黄素的玉米醇溶蛋白(Zein)纳米纤维,即将姜黄素负载在Zein和聚环氧乙烷(Polyethylene oxide,PEO)组成的三种核/壳结构纳米纤维中,并以透射电镜(TEM)、紫外分光(UV)、红外光谱(FT-IR)和XRD等进行相关表征。循环伏安(CV)的释放动力学研究表明在负载量为0.226、0.260、0.264 mg的基础上,姜黄素的封装效率达到96.02%、95.00%、90.15%,与姜黄素对Zein的亲和力略强于PEO一致;通过对姜黄素的电化学特性研究,发现玉米醇溶蛋白包聚环氧乙烷膜的缓释作用最好;借助SEM可以观察到姜黄素释放后纤维膜会出现孔洞,原有的结构遭到破坏;抗菌性研究发现,金黄色葡萄球菌比大肠杆菌的对姜黄素含量更敏感。同轴静电纺丝制得的负载姜黄素纳米纤维可用于功能性食品及生物医用产品的保鲜及贮藏保质。

As a by-product of starch production,zein is widely used in feed and has a low utilization rate.In this experiment,electrospinning technology was used to obtain nanofiber membranes by high-value use of zein,which was used for antibacterial research.The zein nanofibers with core/shell supported curcumin were developed by coaxial electrospinning.Curcumin was supported in three core/shell nanofibers made up of zein and polyethylene oxide(PEO),and characterized by transmission electron microscopy(TEM),UV,FT-IR and XRD.The results of cyclic voltammetry(CV)release kinetics showed that the encapsulation efficiency of curcumin reached 96.02%,95.00%and 90.15%on the basis of loading capacity of 0.226,0.260 and 0.264 mg,which was consistent with the affinity of curcumin to zein slightly stronger than that of PEO.By studying the electrochemical properties of curcumin,it was found that zein coated polyethylene oxide film had the best sustained-release effect;By SEM,it could be observed that after the release of curcumin,the fiber membrane appeared holes and the structure was destroyed.Antimicrobial studies showed that Staphylococcus aureus was more sensitive to curcumin than E.coli.The curcumin loaded nanofibers prepared by coaxial electrospinning could be used for the preservation and storage of functional food and biomedical products.