通过式固相萃取-超高效液相色谱-串联质谱法测定猪肉中多种受体激动剂药物残留

Determination of Multiple Receptor Agonist Drug Residues in Pork by Pass-type SPE and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

建立了猪肉中多种受体激动剂药物残留同时检测的超高效液相色谱-串联质谱方法。样品经酶解后,以酸化乙腈为提取液,PRIME HLB通过式净化的方式,超高效液相色谱-串联质谱法(ultra performance liquid chromatographytandem mass spectrometry,UPLC-MS/MS),电喷雾正离子模式,多反应监测采集(MRM),外标法定量。结果表明,11种受体激动剂在0.1~100μg/L范围内线性关系良好,相关系数在0.9992~0.9999之间,检出限为0.5μg/kg,得到的平均回收率为81.3%~117.6%,相对标准偏差RSD值在0.7%~6.5%之间。该方法过程简单,适用于猪肉中多种受体激动剂药物残留的同时检测。

A method was established for the simultaneous determination of multiple receptor agonist drug residues in pork by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).After enzymatic hydrolysis,the samples were extracted by acidic acetonitrile,and cleaned up by pass-type PRIME HLB,then detected by UPLC-MS/MS in the positive mode and multiple reaction monitoring(MRM)mode,and quantified by the external standard method.Results showed that,good linear relationship were obtained for the 11 multiple receptor agonist drugs in the range of 0.1~100μg/L,with coefficients of determination between 0.9992 and 0.9999.The limits of detection(LOD)for the method were 0.5μg/kg,the average recoveries of all compouds were between 81.3%and 117.6%,with the relative standard deviations varying from 0.7%to 6.5%.The method was simple and would be suitable for simultaneous determination of multiple receptor agonist drug residues in pork.